Document Detail

Enzymatic measurement of choline-containing phospholipids in bile.
MedLine Citation:
PMID:  7017049     Owner:  NLM     Status:  MEDLINE    
An enzymatic method, previously used to determine choline-containing phospholipids in serum, was applied to the estimation of phospholipid concentration in bile. The method combines three enzymatic reactions: a) release of choline by phospholipase D; b) choline oxidation by choline oxidase, a reaction which generates hydrogen peroxide; and c) formation of a red quinone dye by peroxidase. The method is an endpoint, spectrophotometric determination. It is simple, sensitive, and reproducible. Accuracy was demonstrated both by linear recoveries of the choline-containing phospholipids in bile and a good correlation with a chemical determination. No interferences by bile acids and/or cholesterol were observed; bile pigment interference was removed by bleaching with white light. This method which determines lipid choline is simpler than the widely used chemical determination of lipid phosphorus.
D Gurantz; M F Laker; A F Hofmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  22     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1981 Feb 
Date Detail:
Created Date:  1981-08-10     Completed Date:  1981-08-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  373-6     Citation Subset:  IM    
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MeSH Terms
Alcohol Oxidoreductases / metabolism
Bile / analysis*
Bile Acids and Salts
Bile Pigments
Lysophosphatidylcholines / analysis
Phosphatidylcholines / analysis
Phospholipase D / metabolism
Phospholipids / analysis*
Grant Support
Reg. No./Substance:
0/Bile Acids and Salts; 0/Bile Pigments; 0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Phospholipids; EC 1.1.-/Alcohol Oxidoreductases; EC oxidase; EC D

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