| Enzymatic measurement of choline-containing phospholipids in bile. | |
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MedLine Citation:
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PMID: 7017049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An enzymatic method, previously used to determine choline-containing phospholipids in serum, was applied to the estimation of phospholipid concentration in bile. The method combines three enzymatic reactions: a) release of choline by phospholipase D; b) choline oxidation by choline oxidase, a reaction which generates hydrogen peroxide; and c) formation of a red quinone dye by peroxidase. The method is an endpoint, spectrophotometric determination. It is simple, sensitive, and reproducible. Accuracy was demonstrated both by linear recoveries of the choline-containing phospholipids in bile and a good correlation with a chemical determination. No interferences by bile acids and/or cholesterol were observed; bile pigment interference was removed by bleaching with white light. This method which determines lipid choline is simpler than the widely used chemical determination of lipid phosphorus. |
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Authors:
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D Gurantz; M F Laker; A F Hofmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of lipid research Volume: 22 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1981 Feb |
Date Detail:
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Created Date: 1981-08-10 Completed Date: 1981-08-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 373-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Oxidoreductases
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metabolism Bile / analysis* Bile Acids and Salts Bile Pigments Humans Lysophosphatidylcholines / analysis Methods Phosphatidylcholines / analysis Phospholipase D / metabolism Phospholipids / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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AM 21506/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Bile Pigments; 0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Phospholipids; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.3.17/choline oxidase; EC 3.1.4.4/Phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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