| Enzymatic α-glucuronylation of maltooligosaccharides using α-glucuronic acid 1-phosphate as glycosyl donor catalyzed by a thermostable phosphorylase from Aquifex aeolicus VF5. | |
| | |
MedLine Citation:
|
PMID: 22265379 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
This paper describes thermostable phosphorylase-catalyzed α-glucuronylation of maltooligosaccharides for the direct synthesis of anionic oligosaccharides having a glucuronic acid residue at the non-reducing end. When the reaction of α-glucuronic acid 1-phosphate (GlcA-1-P) as a glycosyl donor and maltotriose as a glycosyl acceptor was performed in the presence of thermostable phosphorylase from Aquifex aeolicus VF5, high performance anion exchange chromatography analysis of the reaction mixture suggested the production of a glucuronylated tetrasaccharide, whose structure was also confirmed by the MALDI-TOF MS measurement of the crude products. Furthermore, treatment of the crude products with glucoamylase supported that the α-glucuronic acid unit was positioned at the non-reducing end of the tetrasaccharide and (1)H NMR analysis suggested that it was bound in an α-(1→4)-linkage. When the α-glucuronylation of maltotetraose using GlcA-1-P was conducted, α-glucuronylated oligosaccharides with various degrees of polymerization were produced. On the other hand, the α-glucuronylation of maltotetraose using GlcA-1-P in the presence of potato phosphorylase did not occur at all, indicating no recognition of GlcA-1-P by potato phosphorylase. |
| | |
Authors:
|
Yuta Umegatani; Hironori Izawa; Mutsuki Nawaji; Kazuya Yamamoto; Akiko Kubo; Michiyo Yanase; Takeshi Takaha; Jun-Ichi Kadokawa |
Related Documents
:
|
8478339 - The cortical peptidoglycan from spores of bacillus megaterium and bacillus subtilis is ... 22060589 - Influence of partial replacement of nacl with kcl on lipid fraction of dry fermented sa... 10847609 - Observation of tetrahydrofolylpolyglutamic acid in bacteria cells by matrix-assisted la... 21753219 - Mixture optimization of beef, turkey, and chicken meat for some of the physical, chemic... 18969079 - Photodegradation kinetics of some indolecarboxylic acids and their regulation effects o... 22041099 - Evolution of ph during immersion of meat protein matrices in acidic marinades. 16660539 - 2,4-dichlorophenoxyacetic acid inhibits the outer membrane nadh dehydrogenase of plant ... 7811289 - Identification of 10-kda proguanylin as a major guanylin molecule in human intestine an... 2126249 - Effect of retinoic acid on two glycosyltransferase activities in c6 cultured glioma cells. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-2 |
Journal Detail:
|
Title: Carbohydrate research Volume: - ISSN: 1873-426X ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Conformational analysis of cellobiose by electronic structure theories.
Next Document: Management of respiratory problems in people with neurodegenerative conditions: a narrative review.