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Enzymatic α-glucuronylation of maltooligosaccharides using α-glucuronic acid 1-phosphate as glycosyl donor catalyzed by a thermostable phosphorylase from Aquifex aeolicus VF5.
MedLine Citation:
PMID:  22265379     Owner:  NLM     Status:  Publisher    
This paper describes thermostable phosphorylase-catalyzed α-glucuronylation of maltooligosaccharides for the direct synthesis of anionic oligosaccharides having a glucuronic acid residue at the non-reducing end. When the reaction of α-glucuronic acid 1-phosphate (GlcA-1-P) as a glycosyl donor and maltotriose as a glycosyl acceptor was performed in the presence of thermostable phosphorylase from Aquifex aeolicus VF5, high performance anion exchange chromatography analysis of the reaction mixture suggested the production of a glucuronylated tetrasaccharide, whose structure was also confirmed by the MALDI-TOF MS measurement of the crude products. Furthermore, treatment of the crude products with glucoamylase supported that the α-glucuronic acid unit was positioned at the non-reducing end of the tetrasaccharide and (1)H NMR analysis suggested that it was bound in an α-(1→4)-linkage. When the α-glucuronylation of maltotetraose using GlcA-1-P was conducted, α-glucuronylated oligosaccharides with various degrees of polymerization were produced. On the other hand, the α-glucuronylation of maltotetraose using GlcA-1-P in the presence of potato phosphorylase did not occur at all, indicating no recognition of GlcA-1-P by potato phosphorylase.
Yuta Umegatani; Hironori Izawa; Mutsuki Nawaji; Kazuya Yamamoto; Akiko Kubo; Michiyo Yanase; Takeshi Takaha; Jun-Ichi Kadokawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-2
Journal Detail:
Title:  Carbohydrate research     Volume:  -     ISSN:  1873-426X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
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