| Enzymatic fluorometric microplate assay for quantitative analysis of 3-hydroxybutyric acid in mice plasma. | |
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MedLine Citation:
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PMID: 22449496 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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3-Hydroxybutyric acid (3HB) is a ketone body, and acts as an indicator of energy balance and a central regulator of energy homeostasis. We report the application of a sensitive fluorometric assay for the quantitative determination of 3HB. The assay is based on the oxidation of 3HB by 3HB dehydrogenase, and on the diaphorase resazurin amplifying system. This simple assay enables the measurement of changes in 3HB levels in the blood of normal mice by very small volume sample collection. This assay will therefore be useful for in vivo studies of small animals. |
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Authors:
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Naoyuki Takayama; Tatsuya Ohara; Norio Yamamoto |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-23 |
Journal Detail:
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Title: Analytical biochemistry Volume: - ISSN: 1096-0309 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Food Science Research Center, House Wellness Foods Corporation, 3-20 Imoji, Itami, Hyogo 664-0011, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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