| Enzymatic determination of several D-amino acids using luminol-mediated chemiluminescence. | |
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MedLine Citation:
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PMID: 6141136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for the quantitative determination of several D-amino acids in the range of 0.05-1 nmol per assay (0.25-5 microM) is described. It is insensitive to the presence of excesses of the respective L-amino acids. The assay system employs D-amino-acid oxidase (hog kidney), peroxidase (horse radish) and luminol; the total photon output elicited by the oxidation of the D-amino acids is determined. The different reactivity of individual D-amino acids with D-amino-acid oxidase limits the applicability of the assay. Indications for the usefulness of immobilized enzymes in D-amino-acid analysers are also given. |
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Authors:
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A Hinkkanen; K Decker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hoppe-Seyler's Zeitschrift für physiologische Chemie Volume: 364 ISSN: 0018-4888 ISO Abbreviation: Hoppe-Seyler's Z. Physiol. Chem. Publication Date: 1983 Nov |
Date Detail:
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Created Date: 1984-03-07 Completed Date: 1984-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985060R Medline TA: Hoppe Seylers Z Physiol Chem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 1549-53 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Animals D-Amino-Acid Oxidase / metabolism* Horseradish Peroxidase / metabolism Kidney / enzymology Kinetics Luminescent Measurements Luminol Stereoisomerism Swine |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 521-31-3/Luminol; EC 1.11.1.-/Horseradish Peroxidase; EC 1.4.3.3/D-Amino-Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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