Document Detail


Enzymatic determination of several D-amino acids using luminol-mediated chemiluminescence.
MedLine Citation:
PMID:  6141136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the quantitative determination of several D-amino acids in the range of 0.05-1 nmol per assay (0.25-5 microM) is described. It is insensitive to the presence of excesses of the respective L-amino acids. The assay system employs D-amino-acid oxidase (hog kidney), peroxidase (horse radish) and luminol; the total photon output elicited by the oxidation of the D-amino acids is determined. The different reactivity of individual D-amino acids with D-amino-acid oxidase limits the applicability of the assay. Indications for the usefulness of immobilized enzymes in D-amino-acid analysers are also given.
Authors:
A Hinkkanen; K Decker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hoppe-Seyler's Zeitschrift für physiologische Chemie     Volume:  364     ISSN:  0018-4888     ISO Abbreviation:  Hoppe-Seyler's Z. Physiol. Chem.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-03-07     Completed Date:  1984-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985060R     Medline TA:  Hoppe Seylers Z Physiol Chem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  1549-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Animals
D-Amino-Acid Oxidase / metabolism*
Horseradish Peroxidase / metabolism
Kidney / enzymology
Kinetics
Luminescent Measurements
Luminol
Stereoisomerism
Swine
Chemical
Reg. No./Substance:
0/Amino Acids; 521-31-3/Luminol; EC 1.11.1.-/Horseradish Peroxidase; EC 1.4.3.3/D-Amino-Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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