Document Detail


Enzymatic determination of free carnitine in milk and infant formulas.
MedLine Citation:
PMID:  7703730     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple spectrophotometric assay for free carnitine in milk and supplemented infant formulas has been developed. After acid extraction, carnitine is measured enzymatically through its reaction with carnitine acetyltransferase coupled with acetyl coenzyme A and dithiobenzoate (DTNB). The manually performed method is rapid, accurate, and convenient for routine quality-control analysis of infant formulas. The chemistry is also suitable for automation, for greatly enhanced throughput.
Authors:
H E Indyk; D C Woollard
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  78     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1995 Jan-Feb
Date Detail:
Created Date:  1995-05-11     Completed Date:  1995-05-11     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  69-74     Citation Subset:  IM    
Affiliation:
Anchor Products, Waitoa, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Acetyl Coenzyme A / chemistry
Animals
Buffers
Carnitine / analysis*,  chemistry
Carnitine O-Acetyltransferase / chemistry
Humans
Indicators and Reagents
Infant
Infant Food / analysis*
Milk / chemistry*
Oxidation-Reduction
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Buffers; 0/Indicators and Reagents; 541-15-1/Carnitine; 72-89-9/Acetyl Coenzyme A; EC 2.3.1.7/Carnitine O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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