| Enzymatic detection of d: -amino acids. | |
| | |
MedLine Citation:
|
PMID: 21956570 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
D: -Amino acids play several key roles and are widely diffused in living organisms, from bacteria (in which D: -alanine is a component of the cell wall) to mammals (where D: -serine is involved in glutamatergic neurotransmission in the central nervous system). The study of the biological processes involving D: -amino acids and their use as clinical or biotechnological biomarkers requires reliable methods of quantifying them. Although "traditional" analytical techniques have been (and still are) employed for such tasks, enzymatic assays based on enzymes which possess a strict stereospecificity (i.e., that are only active on the D: -enantiomers of amino acids) allowed the set-up of low-cost protocols with a high sensitivity and selectivity and suitable for determining the D: -amino acid content of complex biological samples. The most exploited enzyme in these assays is D: -amino acid oxidase, a flavoenzyme that exclusively oxidizes D: -amino acids and possesses with a broad substrate specificity and a high kinetic efficiency. |
| | |
Authors:
|
Gianluca Molla; Luciano Piubelli; Federica Volontè; Mirella S Pilone |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 794 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
|
Created Date: 2011-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 273-89 Citation Subset: IM |
Affiliation:
|
Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, Varese, Italy, gianluca.molla@uninsubria.it. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Estimation of chronological age from the racemization rate of L: - and d: -aspartic Acid: how to com...
Next Document: An Enzymatic-HPLC Assay to Monitor Endogenous D: -Serine Release from Neuronal Cultures.