Document Detail


Enzymatic detection of d: -amino acids.
MedLine Citation:
PMID:  21956570     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
D: -Amino acids play several key roles and are widely diffused in living organisms, from bacteria (in which D: -alanine is a component of the cell wall) to mammals (where D: -serine is involved in glutamatergic neurotransmission in the central nervous system). The study of the biological processes involving D: -amino acids and their use as clinical or biotechnological biomarkers requires reliable methods of quantifying them. Although "traditional" analytical techniques have been (and still are) employed for such tasks, enzymatic assays based on enzymes which possess a strict stereospecificity (i.e., that are only active on the D: -enantiomers of amino acids) allowed the set-up of low-cost protocols with a high sensitivity and selectivity and suitable for determining the D: -amino acid content of complex biological samples. The most exploited enzyme in these assays is D: -amino acid oxidase, a flavoenzyme that exclusively oxidizes D: -amino acids and possesses with a broad substrate specificity and a high kinetic efficiency.
Authors:
Gianluca Molla; Luciano Piubelli; Federica Volontè; Mirella S Pilone
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  794     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-09-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-89     Citation Subset:  IM    
Affiliation:
Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, Varese, Italy, gianluca.molla@uninsubria.it.
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