Document Detail


Enzymatic and antigenic analyses of strains of Mycobacterium bovis, M. bovis BCG, and M. tuberculosis.
MedLine Citation:
PMID:  7765849     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Strains of Mycobacterium bovis, M. bovis BCG, and M. tuberculosis, including a so-called Canetti strain, were analyzed by means of two-dimensional immunoelectrophoresis (2D-IE), 2D-IE combined with enzyme staining, and multilocus enzyme electrophoresis (MEE). The results demonstrated a close antigenic and enzymatic resemblance among all the strains tested, even though the BCG strains could be divided into two groups based on the presence of one precipitinogen. Eight of the precipitinogens were shown to correspond to enzymes in M. bovis BCG and 10 in M. tuberculosis. Thus, catalase, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, and several others were identified. By means of MEE the strains of M. tuberculosis, M. bovis, and M. bovis BCG could be differentiated. The analyses further indicated that the M. tuberculosis strain Canetti was more closely related to M. bovis than to M. tuberculosis.
Authors:
R Ohman; M Ridell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current microbiology     Volume:  30     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-03-30     Completed Date:  1995-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  161-5     Citation Subset:  B    
Affiliation:
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / isolation & purification*
Catalase / isolation & purification
Immunoelectrophoresis, Two-Dimensional
Mycobacterium bovis / classification,  enzymology*,  immunology*
Mycobacterium tuberculosis / classification,  enzymology*,  immunology*
Oxidoreductases / isolation & purification
Peroxidase / isolation & purification
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; EC 1.-/Oxidoreductases; EC 1.11.1.6/Catalase; EC 1.11.1.7/Peroxidase

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