Document Detail

Enzymatic analysis of the gingival crevicular fluid in hypoxia of high altitude (Everest).
MedLine Citation:
PMID:  17897492     Owner:  NLM     Status:  MEDLINE    
The aim of this study is to determine the qualitative and quantitative changes of alkaline phosphatase (ALP) that occur in the Gingival Crevicular Fluid (GCF) in hypobaric-hypoxic conditions (high altitude). Hypoxia affects systemic adaptation responses in different organs. We examined 17 Caucasians subjects, of whom 13 were mountain climbers (1 female and 12 males), and 4 Tibetans (2 females and 2 males) following exposure to the hypoxia environment of high altitude. The study was conducted at different altitudes (0 m control, 1000 m, 5200 m above sea level) on Mount Everest. The protocol consisted of withdrawing crevicular fluid through the use of cones made of endodontic paper size 30 sectioned to 15 mm from the apex, inserted for 30 seconds in the gingival sulcus (about 2 mm). The analyzed sites were the mesial and distal, buccal and palatal of tooth 1.1 and 2.1. Blood exams were performed on the subjects using I-Stat, furnishing analysis in real time (about 2 mins). In agreement with other results reported in literature, in all the subjects we found an increase in the hematocrit and hemoglobin with a large range of values between them, and with significant differences, as analysed with the Fisher, Scheffe and Bonferroni/Dunn statistical methods. The enzymatic analysis of the GFC showed an increase of the levels of ALP at each altitude studied. With this preliminary study we show that hypoxic environment determines not only the well known cardiovascular systemic responses, but also crevicular fluid adaptation.
P Trentini; M Ferrante; M Dolci; L Ciavarelli; A Tondi; G Spoto
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of immunopathology and pharmacology     Volume:  20     ISSN:  0394-6320     ISO Abbreviation:  Int J Immunopathol Pharmacol     Publication Date:    2007 Jan-Mar
Date Detail:
Created Date:  2007-09-27     Completed Date:  2008-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911335     Medline TA:  Int J Immunopathol Pharmacol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
Department of Stomatology and Oral Sciences, Medical and Dental Schools, University of Chieti-Pescara, Chieti, Italy.
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MeSH Terms
Alkaline Phosphatase / metabolism*
Anoxia / enzymology*
Gingival Crevicular Fluid / enzymology*
Reg. No./Substance:
EC Phosphatase

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