| Enzymatic amplification detection of DNA based on "molecular beacon" biosensors. | |
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MedLine Citation:
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PMID: 18304797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We described a novel electrochemical DNA biosensor based on molecular beacon (MB) probe and enzymatic amplification protocol. The MB modified with a thiol at its 5' end and a biotin at its 3' end was immobilized on the gold electrode through mixed self-assembly process. Hybridization events between MB and target DNA cause the conformational change of the MB, triggering the attached biotin group on the electrode surface. Following the specific interaction between the conformation-triggered biotin and streptavidin-horseradish peroxidase (HRP), subsequent quantification of DNA was realized by electrochemical detection of enzymatic product in the presence of substrate. The detection limit is obtained as low as 0.1nM. The presented DNA biosensor has good selectivity, being able to differentiate between a complementary target DNA sequence and one containing G-G single-base mismatches. |
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Authors:
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Xun Mao; Jianhui Jiang; Xiangmin Xu; Xia Chu; Yan Luo; Guoli Shen; Ruqin Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-24 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 23 ISSN: 0956-5663 ISO Abbreviation: Biosens Bioelectron Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-14 Completed Date: 2008-08-04 Revised Date: 2009-07-14 |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 1555-61 Citation Subset: IM |
Affiliation:
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State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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instrumentation*,
methods DNA / chemistry*, genetics* Electrochemistry / instrumentation*, methods Equipment Design Equipment Failure Analysis Horseradish Peroxidase / chemistry* Molecular Probe Techniques / instrumentation* Nucleic Acid Amplification Techniques / instrumentation*, methods Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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