Document Detail


Enzymatic alterations in single type IIB skeletal muscle fibers with inactivity and exercise in 12- and 30-month-old rats.
MedLine Citation:
PMID:  12602568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the present study was to examine the effects of aging, inactivity and weight-bearing exercise on fast-twitch single Type IIB skeletal muscle fibers from the superficial region of the lateral head of the gastrocnemius (Type IIB fibers). Specifically, this study compared the biochemical properties of Type IIB fibers after 7 days of hindlimb unweighting (HU), 7 days of HU with intermittent weight-bearing (HU-Ex), and cage control (C) from adult and aged Fischer 344 Brown Norway F1 Hybrid rats (12- and 30-month old). Biochemical measurements included total lactate dehydrogenase (LDH) and beta-hydroxy-acyl-coenzyme A dehydrogenase activities (BHAD), expressed in nmoles/microg/hr dry weight. Fiber-typing for myosin heavy chain isoform was determined by SDS-PAGE. With age, LDH activity in Type IIB fibers decreased from 52.0 +/- 3.4 nmoles/microg/hr (12-month old) to 39.5 +/- 2.9 nmoles/microg/hr (30-month old). Following HU, LDH activity of single Type IIB fibers increased by 22% (52.0 +/- 3.4 to 66.4 +/- 3.2 nmoles/microg/hr) in the 12-month-old animals, whereas no difference was observed with HU in the Type IIB fibers of the 30-month-old animals. Following HU-Ex, LDH activity of Type IIB fibers in the 12-month-old animals was not significantly different from that of Type IIB fibers from HU animals, whereas a significant increase was observed (38.1 +/- 2.9 to 51.8 +/- 3.1 nmoles/microg/hr) in Type IIB fibers of 30-month-old animals, for HU and HU-Ex, respectively. Analysis of variance revealed an interaction between age and condition, indicating that Type IIB fibers from adult and aged animals have a different biochemical response to inactivity. The enzyme activities for BHAD were not different between the experimental conditions. The results demonstrate that the total LDH enzyme activities of the Type IIB fibers decrease with age, suggesting an age-related shift in the biochemical profile. Further, single skeletal muscle fiber adaptation is age-dependent.
Authors:
Jon J Groskreutz; LaDora V Thompson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Aging clinical and experimental research     Volume:  14     ISSN:  1594-0667     ISO Abbreviation:  Aging Clin Exp Res     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2003-02-26     Completed Date:  2003-08-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101132995     Medline TA:  Aging Clin Exp Res     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  347-53     Citation Subset:  IM; S    
Affiliation:
Program in Physical Therapy, Department of Physical Medicine and Rehabilitation, School of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxyacyl CoA Dehydrogenases / metabolism*
Aging / physiology*
Animals
Body Weight
Hindlimb Suspension / physiology
L-Lactate Dehydrogenase / metabolism*
Male
Muscle Fibers, Fast-Twitch / enzymology*
Muscle, Skeletal / cytology,  physiology*
Organ Size
Physical Exertion / physiology*
Rats
Rats, Inbred F344
Chemical
Reg. No./Substance:
EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.1.1.35/3-Hydroxyacyl CoA Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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