Document Detail


Enzymatic improvement in the polyphenol extractability and antioxidant activity of green tea extracts.
MedLine Citation:
PMID:  23291774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study describes increases in extraction efficiency and the bioconversion of catechins after treatment with several commercial enzymes. Tannase was also used to improve the anti-radical activities of green tea extracts. Enzymatic treatment with various commercial enzymes was introduced to improve the extraction efficiency of polyphenols. The total polyphenol, flavonoid, and catechin contents and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the green tea extract treated with Viscozyme (VG) were significantly higher than those treated with other commercial enzymatic extractions (p<0.05). More than 95% of the epigallocatechingallate (EGCG) and of the epicatechingallate (ECG) was hydrolyzed to epigallocatechin (EGC) and to epicatechin (EC) in successive 20 min treatments with Viscozyme and tannase (TG). Due to its hydrolytic activity, treatment involving tannase resulted in a significant release of gallic acid (GA), EGC, and EC, leading to greater radical scavenging activities. Regarding the IC(50) values of the DPPH and 2,2-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, the green tea extract treated with TG showed values of 131.23 and 28.83 µg/mL, VG showed values of 224.70 and 32.54 µg/mL, and normal green tea extract (NG) showed values of 241.11 and 66.27 µg/mL, respectively. These results indicate that successive treatment with Viscozyme and tannase improves the extraction efficiency of polyphenols and increases radical scavenging activities.
Authors:
Yang-Hee Hong; Eun Young Jung; Yooheon Park; Kwang-Soon Shin; Tae Young Kim; Kwang-Won Yu; Un Jae Chang; Hyung Joo Suh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  77     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-07-05     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  22-9     Citation Subset:  IM    
Affiliation:
Research Institute of Health and Sciences and Department of Food and Nutrition, Korea University, Seoul, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / isolation & purification*
Biphenyl Compounds / antagonists & inhibitors
Carboxylic Ester Hydrolases / chemistry
Catechin / analogs & derivatives*,  isolation & purification*
Cellulases / chemistry
Endo-1,4-beta Xylanases / chemistry
Fungal Proteins / chemistry
Gallic Acid / isolation & purification*
Glycoside Hydrolases / chemistry
Picrates / antagonists & inhibitors
Plant Extracts / chemistry*
Plant Leaves / chemistry*
Sulfonic Acids / antagonists & inhibitors
Tea / chemistry*
Thiazoles / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Biphenyl Compounds; 0/Fungal Proteins; 0/Picrates; 0/Plant Extracts; 0/Sulfonic Acids; 0/Tea; 0/Thiazoles; 149-91-7/Gallic Acid; 154-23-4/Catechin; 1617-55-6/gallocatechol; 1898-66-4/2,2-diphenyl-1-picrylhydrazyl; 28752-68-3/2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.20/tannase; EC 3.2.1.-/Cellulases; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/hemicellulase; EC 3.2.1.8/Endo-1,4-beta Xylanases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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