| Environmental and synthetic sulphydryl group inhibitors: effects on bioluminescence and respiration in Vibrio fischeri. | |
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MedLine Citation:
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PMID: 17411357 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Elemental sulphur (as S0 and S8) is abundant in anaerobic sediments and soil, and is highly toxic in the Vibrio fischeri bioluminescence test. This mode of S0 action remains uncertain. The objective of this research was the analysis of the toxic effects of S0 on bioluminescence and respiration in V. fischeri, in joint action with N-ethylmaleimide (NEM) or 2,4-dithio-DL-threitol (DTT), which are -SH group inhibiting and maintaining synthetic agents, respectively. Non-toxic DTT immediately protected cell bioluminescence against S0 inhibition at low (5.5ppb) and high (55ppb) concentrations of S0, whilst restoration of the inhibitory effect of S0 took up to 30 minutes. NEM (62.5ppb) diminished cell bioluminescence by up to 50% after 5 minutes, but after 60 minutes, the inhibition reached 100%. DTT restored the bioluminescence function inhibited in vivo and in vitro by S0 and NEM. Enhancement of cell respiration by up to 20% and 33% was observed at 2.2ppm of S0 and 36.8ppm of 2,4-dinitrophenol (2,4-DNP; an uncoupler of oxidative phosphorylation), respectively; whilst NEM (3.1ppm) caused a reduction of up to 40%. This comparative analysis confirmed that S0 has multiple modes of action--it acts as both an -SH group inhibitor and an uncoupler of oxidative phosphorylation in V. fischeri cells. |
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Authors:
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Virginija Kalciene; Anolda Cetkauskaite |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Alternatives to laboratory animals : ATLA Volume: 35 ISSN: 0261-1929 ISO Abbreviation: Altern Lab Anim Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-04-06 Completed Date: 2007-05-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8110074 Medline TA: Altern Lab Anim Country: England |
Other Details:
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Languages: eng Pagination: 93-100 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, Vilnius University, Vilnius, Lithuania. virginija.kalciene@gf.vu.lt |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dinitrophenol
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pharmacology Chemiluminescent Measurements Dithiothreitol / pharmacology Dose-Response Relationship, Drug Drug Antagonism Drug Combinations Ethylmaleimide / toxicity* Luminescence* Oxygen Consumption / drug effects* Sulfhydryl Reagents / toxicity* Sulfur / toxicity* Vibrio fischeri / drug effects*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Drug Combinations; 0/Sulfhydryl Reagents; 128-53-0/Ethylmaleimide; 3483-12-3/Dithiothreitol; 51-28-5/2,4-Dinitrophenol; 7704-34-9/Sulfur |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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