| Environmental salinity regulates the in vitro production of [3H]-1,25-dihydroxyvitamin D3 and [3H]-24,25 dihydroxyvitamin D3 in rainbow trout (Oncorhynchus mykiss). | |
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MedLine Citation:
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PMID: 17292365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have shown that specific binding of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) to enterocyte basolateral membranes (BLM), as well as circulating concentrations, is affected in response to changes in environmental salinity. It is not known if the production of 1,25(OH)2D3 and 24,25(OH)2D3 is affected by environmental salinity. The aim of the present study was to measure the in vitro production of [3H]-1,25(OH)2D3 and [3H]-24,25(OH)2D3 in fresh water (FW) and after 1, 2, 3, and 7 days after transfer to seawater (SW). Pooled sub-cellular fractions (mitochondria and microsomes) from liver or kidney was incubated with [3H]-25(OH)D3 and the produced metabolites were separated using HPLC. Hepatic production of [3H]-1,25(OH)2D3 was decreased after 24h in SW. This was followed by an up-regulation after 48h and a second, slower decrease in production rate which leveled out after 7 days in SW. The production rate in SW was lower than the original rate in FW-adapted fish. For hepatic [3H]-24,25(OH)2D3 production the pattern was reversed. Renal production of [3H]-24,25(OH)2D3 increased significantly during the period of SW acclimation. These results suggest that environmental salinity regulates the production rate of the two antagonizing calcium regulatory hormones; 1,25(OH)2D3 and 24,25(OH)2D3. This gives further evidence to the hypothesis that there is a physiological regulation and a differentiated importance of 1,25(OH)2D3 and 24,25(OH)2D3 in relation to environmental calcium concentrations. |
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Authors:
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Henrik Sundh; Dennis Larsson; Kristina Sundell |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2006-12-30 |
Journal Detail:
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Title: General and comparative endocrinology Volume: 152 ISSN: 0016-6480 ISO Abbreviation: Gen. Comp. Endocrinol. Publication Date: 2007 Jun-Jul |
Date Detail:
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Created Date: 2007-06-25 Completed Date: 2007-08-15 Revised Date: 2008-08-16 |
Medline Journal Info:
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Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 252-8 Citation Subset: IM |
Affiliation:
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Fish Endocrinology Laboratory, Department of Zoology, Göteborg University, Box 463, SE-405 30 Göteborg, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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24,25-Dihydroxyvitamin D 3
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metabolism* 25-Hydroxyvitamin D3 1-alpha-Hydroxylase / metabolism Animals Calcitriol / metabolism* Calcium / metabolism* Environment Female Fresh Water Kidney / enzymology Liver / enzymology Male Oncorhynchus mykiss / metabolism* Seawater Steroid Hydroxylases / metabolism Tritium / diagnostic use Water-Electrolyte Balance / physiology* |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 32222-06-3/Calcitriol; 40013-87-4/24,25-Dihydroxyvitamin D 3; 7440-70-2/Calcium; EC 1.14.-/25-Hydroxyvitamin D3 1-alpha-Hydroxylase; EC 1.14.-/Steroid Hydroxylases; EC 1.14.13.-/vitamin D 24-hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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