| Environmental monitoring using acetylcholinesterase inhibition in vitro. A case study in two Mexican lagoons. | |
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MedLine Citation:
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PMID: 11460718 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cholinesterase inhibition is considered a specific biomarker of exposure and effect for organophosphorous pesticides. Its use for monitoring has been hindered, particularly in tropical countries where organophosphates are widely used for malaria and dengue control, because of the frequent lack of suitable controls. An in vitro technique is proposed as a biochemical method for monitoring pollutant mixtures in sediment toxicity tests. Brain homogenate from the fish Oreochromis niloticus is used as the enzyme source. Optimum incubation time, extraction solvent and effect of crude oil on acetylcholinesterase (AChE) are reported. The method described was used in sediments from two Mexican lagoons, located in an oil extraction area where pesticides are used in agriculture and vector control campaigns. AChE inhibitions from 3 to 21% were found in these lagoons, even in the presence of high concentrations of petroleum. |
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Authors:
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G Rodríguez-Fuentes; G Gold-Bouchot |
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Publication Detail:
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Type: Case Reports; In Vitro; Journal Article |
Journal Detail:
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Title: Marine environmental research Volume: 50 ISSN: 0141-1136 ISO Abbreviation: Mar. Environ. Res. Publication Date: 2000 Jul-Dec |
Date Detail:
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Created Date: 2001-07-19 Completed Date: 2001-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9882895 Medline TA: Mar Environ Res Country: England |
Other Details:
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Languages: eng Pagination: 357-60 Citation Subset: IM |
Affiliation:
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CINVESTAV, Unidad Mérida Departamento de Recursos del Mar, Apdo. Postal 73 Cordemex, Mérida Yucatan 97310, Mexico. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholinesterase
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metabolism* Animals Aquaculture Brain / enzymology Cholinesterase Inhibitors / toxicity* Environmental Monitoring / methods* Fresh Water Geologic Sediments Mexico Soil Pollutants / toxicity Tilapia / metabolism* Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Cholinesterase Inhibitors; 0/Soil Pollutants; 0/Water Pollutants, Chemical; EC 3.1.1.7/Acetylcholinesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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