Document Detail

Environmental induction of tumor phenotype in a putative Kaposi sarcoma progenitor cell.
MedLine Citation:
PMID:  8207969     Owner:  NLM     Status:  MEDLINE    
Many features of AIDS-related Kaposi sarcoma (AIDS-KS), e.g., multifocal lesional presentation at sites perfused by the microvasculature, suggest that AIDS-KS is initially a hyperplasia that subsequently progresses to a neoplasia. We propose that the unique AIDS environment, which contains high levels of circulating factors such as viral cytokines, is key in initiating the KS lesion. Further, we maintain that due to their physiological function, human microvascular endothelial cells (HMECs) are both likely target cells for the AIDS-related cytokines, and are putative AIDS-KS progenitor cells. Previously, we have shown that as a component of HMEC transition between proliferative and differentiated growth, HMECs modulate their nucleotide and glutathione levels. After attaining contact inhibition, HMECs enter a state of differentiation, which is characterized by cellular entrance into a G0, quiescent growth state, a decrease in cellular bioenergetic profiles, and spontaneous formation of microtubules. In contrast, when cultured in a "KS milieu", HMECs fail to differentiate. Instead, the "KS milieu" cultured cells assume a "growth relaxed" phenotype and demonstrate a lack of contact inhibition, loss of anchorage dependence, and retention of a "proliferative" bioenergetic profile despite culture confluence. Our results imply both that HMECs are responsive to AIDS-related cytokines, and that the local environment is key to instigating a relaxation of cellular growth controls.
S R Mallery; L E Lantry; A Hegtvedt; A Lazo; L C Titterington; B L Hout; G P Brierley; R E Stephens
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lymphology     Volume:  27     ISSN:  0024-7766     ISO Abbreviation:  Lymphology     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-07-12     Completed Date:  1994-07-12     Revised Date:  2006-11-03    
Medline Journal Info:
Nlm Unique ID:  0155112     Medline TA:  Lymphology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  45-8     Citation Subset:  IM; X    
College of Dentistry, Department of Pathology, Ohio State University, Columbus.
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MeSH Terms
AIDS-Related Complex / pathology*
Cell Differentiation
Cell Transformation, Neoplastic
Endothelium, Vascular / cytology
Sarcoma, Kaposi / pathology*
Stem Cells / pathology
Tumor Cells, Cultured

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