Document Detail


Environmental factors that control microbial perchlorate reduction.
MedLine Citation:
PMID:  12200296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As part of a study to elucidate the environmental parameters that control microbial perchlorate respiration, we investigated the reduction of perchlorate by the dissimilatory perchlorate reducer Dechlorosoma suillum under a diverse set of environmental conditions. Our results demonstrated that perchlorate reduction by D. suillum only occurred under anaerobic conditions in the presence of perchlorate and was dependent on the presence of molybdenum. Perchlorate reduction was dependent on the presence of the enzyme chlorite dismutase, which was induced during metabolism of perchlorate. Anaerobic conditions alone were not enough to induce expression of this enzyme. Dissolved oxygen concentrations less than 2 mg liter(-1) were enough to inhibit perchlorate reduction by D. suillum. Similarly to oxygen, nitrate also regulated chlorite dismutase expression and repressed perchlorate reduction by D. suillum. Perchlorate-grown cultures of D. suillum preferentially reduced nitrate in media with equimolar amounts of perchlorate and nitrate. In contrast, an extended (40 h) lag phase was observed if a similar nitrate-perchlorate medium was inoculated with a nitrate-grown culture. Perchlorate reduction commenced only when nitrate was completely removed in either of these experiments. In contrast to D. suillum, nitrate had no inhibitory effects on perchlorate reduction by the perchlorate reducer Dechloromonas agitata strain CKB. Nitrate was reduced to nitrite concomitant with perchlorate reduction to chloride. These studies demonstrate that microbial respiration of perchlorate is significantly affected by environmental conditions and perchlorate reduction is directly dependent on bioavailable molybdenum and the presence or absence of competing electron acceptors. A microbial treatment strategy can achieve and maintain perchlorate concentrations below the recommended regulatory level, but only in environments in which the variables described above can be controlled.
Authors:
Swades K Chaudhuri; Susan M O'Connor; Ruth L Gustavson; Laurie A Achenbach; John D Coates
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-29     Completed Date:  2002-10-11     Revised Date:  2014-03-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4425-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Environment*
Gene Expression
Molybdenum / pharmacology
Nitrates / pharmacology
Oxidation-Reduction / drug effects
Oxidoreductases / genetics,  metabolism*
Oxygen / metabolism
Perchlorates / metabolism*
Proteobacteria / drug effects,  enzymology,  genetics,  metabolism*
Sodium Compounds / metabolism*
Chemical
Reg. No./Substance:
0/Nitrates; 0/Perchlorates; 0/Sodium Compounds; 81AH48963U/Molybdenum; 97F4MTY3VA/sodium perchlorate; EC 1.-/Oxidoreductases; EC 1.13.11.49/chlorite dismutase; S88TT14065/Oxygen
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