| Environmental Tobacco Smoke Exposure During Intrauterine Period Promotes Granulosa Cell Apoptosis: A prospective, randomized study. | |
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MedLine Citation:
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PMID: 22468922 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Objective: To evaluate the intrauterine effect of cigarette smoke on cell death and DNA damage in follicular cells of fetal ovarian tissue. Methods: A prospective, randomized study was conducted with 25 female wistar-albino rats. The rats were randomized either to be exposed to cigarette smoke or to room air, initiating from proestrous period and during pregnancy. Newborn female rats were categorized as group1 (n=24) that had been exposed to cigarette smoke during intrauterine life and group2 (n=7) that had been exposed to room air during intrauterine life. Bilateral ooferectomies were performed on the second week of their life. TUNEL (in-situ Terminal Deoxynucleotidyl-Transferase Mediated dUTP-Nick-End Labeling) immunofluorescent staining and immunohistochemical analyses with caspase-3 were used for detection of DNA damage and apoptosis. Primary outcomes were apoptotic index and immunohistochemical scores (HSCORE). Secondary outcomes were ovarian follicle counts and birth weights of newborn rats. Results: There was a significant increase of HSCORE and apoptotic index in group1.Increased immunofluorescent staining; evaluating DNA damage, with TUNEL method was observed in granulosa cells in group1. Conclusions: Intrauterine exposure to cigarette smoke diminishes ovarian reserve of female offspring, raising the concern about the generational impact of maternal smoking on ovarian function in the human. |
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Authors:
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Sevtap Kilic; Beril Yuksel; Nese Lortlar; Semra Sertyel Bio; Tarik Aksu; Sertac Batioglu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-3 |
Journal Detail:
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Title: The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians Volume: - ISSN: 1476-4954 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101136916 Medline TA: J Matern Fetal Neonatal Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Dr. Zekai Tahir Burak Women's Health, Education and Research Hospital, Department of Reproductive Endocrinology and IVF, Cebeci/ANKARA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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