Document Detail

Envelope glycoproteins of hantavirus can mediate cell-cell fusion independently.
MedLine Citation:
PMID:  17619252     Owner:  NLM     Status:  MEDLINE    
Hantaviruses (HTVs) are enveloped viruses and can induce low PH-dependent cell fusion. In this report we molecularly cloned viral glycoproteins (GPs) cDNA and nucleocapsid (NP) cDNA of two strains of Hantaan virus and one strain of Seoul virus and expressed in Vero E6 cells under control of a CMV promoter. The examinations of viral gene expressions were carried out by IFA and immune-precipitation. After treatment with low PH (PH 5.8) medium the syncytium were observed in the cells transfected with the GPs clones while in the cells transfected with the NP clones we did not find this phenomenon. Furthermore cotransfection of the NP and GPs did not enhance fusion activity. Treatment with anti-GP monoclonal antibodies could inhibit fusion activity whereas the antibodies against NP could not. These results indicated that GPs can mediate cell-cell fusion independently.
Feng Zheng; Lixian Ma; Lihua Shao; Gang Wang; Fengzhe Chen; Ying Zhang; Song Yang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The new microbiologica     Volume:  30     ISSN:  1121-7138     ISO Abbreviation:  New Microbiol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-07-10     Completed Date:  2007-08-21     Revised Date:  2011-02-25    
Medline Journal Info:
Nlm Unique ID:  9516291     Medline TA:  New Microbiol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  101-7     Citation Subset:  IM    
Qilu Hospital of Shandong University, Jinan, Shandong Province, China.
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MeSH Terms
Cell Fusion*
Cercopithecus aethiops
Cloning, Molecular
Fluorescent Antibody Technique, Indirect
Gene Expression
Giant Cells / cytology
Hantaan virus / physiology*
Hydrogen-Ion Concentration
Nucleocapsid Proteins / genetics,  physiology*
Seoul virus / physiology*
Vero Cells
Viral Envelope Proteins / genetics,  physiology*
Reg. No./Substance:
0/Nucleocapsid Proteins; 0/Viral Envelope Proteins

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