Document Detail


Enumeration and viability of rare cells in a microfluidic disk via positive selection approach.
MedLine Citation:
PMID:  22813710     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Recent studies have shown that specific rare cells in the blood can serve as an indicator of cancer prognosis, among other purposes. This paper demonstrates the concept of separating and detecting rare cells from peripheral blood mononuclear cells via an economical microfluidic disk with a model system. MCF7, labeled with magnetic beads, was used to simulate circulating tumor cell as a target. Jurkat Clone E6-1 was used to simulate leukocytes or other cells abundant in human blood. A tailored multi-stage magnet maximized the magnetic field to ensure optimal trapping efficiency. Results indicate that the yield of detected MCF7 was consistent at approximately 80% when fewer than hundreds of MCF7 cells were mixed in greater than one million Jurkat cells. The 80% yield also held for ten MCF7 in one hundred million Jurkat (rarity of 10 (7)). Compared to the results from autoMACS, the performance was at least 20% higher and was more independent of the number of Jurkat. The viability of the enriched cells was around 90±20%, showing this method caused little damage to trapped cells. The microfluidic disk should be applicable for separation and detection of various rare cells, such as circulating tumor cells and circulating endothelial cells in human blood.
Authors:
Ken-Chao Chen; Yu-Cheng Pan; Chen-Lin Chen; Andrew M Wo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-16
Journal Detail:
Title:  Analytical biochemistry     Volume:  -     ISSN:  1096-0309     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taiwan.
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