Document Detail


Enumeration of food-borne Clostridium perfringens in egg yolk-free tryptose-sulfite-cycloserine agar.
MedLine Citation:
PMID:  4363368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The SFP (Shahidi-Ferguson perfringens), TSC (tryptose-sulfite-cycloserine), EY (egg yolk)-free TSC, and OPSP (oleandomycin-polymyxin-sulfadiazine perfringens) agars have been tested for their suitability to enumerate Clostridium perfringens in naturally contaminated foods. Complete recoveries of C. perfringens were obtained in each of the four media, but only the TSC and EY-free TSC agars were sufficiently selective to ensure subsequent confirmatory tests without interference from facultative anaerobes. Because of some disadvantages associated with the use of egg yolk, EY-free TSC agar is recommended for enumeration of C. perfringens in foods. Several conditions for convenient shipment of foods and C. perfringens isolates with minimum loss of viability have been tested. The highest viable counts were preserved when foods were mixed 1:1 (wt/vol) with 20% glycerol and kept in a container with dry ice. Isolated C. perfringens strains remained viable for at least 2 weeks at ambient temperatures on blood agar slopes with a 2% agar overlay in screw-cap culture tubes.
Authors:
A H Hauschild; R Hilsheimer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  27     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1974 Mar 
Date Detail:
Created Date:  1974-06-26     Completed Date:  1974-06-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  521-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agar*
Anaerobiosis
Animals
Bacteriological Techniques
Blood
Cell Count
Cell Survival
Clostridium perfringens / immunology,  isolation & purification*
Culture Media
Cycloserine
Food Contamination
Food Microbiology*
Food Preservation
Glycerol
Hemolysis
Sheep
Spores, Bacterial / isolation & purification
Sulfites
Temperature
Chemical
Reg. No./Substance:
0/Culture Media; 0/Sulfites; 56-81-5/Glycerol; 68-41-7/Cycloserine; 9002-18-0/Agar
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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