Document Detail


Enumeration and detection of acetic acid bacteria by real-time PCR and nested PCR.
MedLine Citation:
PMID:  16451189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acetic acid bacteria play a negative role in wine making because they increase the volatile acidity of wines. They can survive in the various phases of alcoholic fermentation and it is very important to control their presence and ulterior development. The main objective of the present work is to test fast, sensitive and reliable techniques such as real-time PCR (rt-PCR) and nested PCR for enumerating and detecting the presence of this bacterial group without plating. Primers were designed on the basis of the available 16S rRNA gene sequences and tested successfully with reference acetic acid bacteria strains. The usefulness of rt-PCR was demonstrated by comparing the results with traditional techniques (colony and microscope counting). The results were similar with all the techniques. Optimized rt-PCR enabled numbers between 10(7) and 10(1) cells mL(-1) to be enumerated, while nested PCR detected less than 10 cells mL(-1). Although this latter technique cannot be used for enumeration, it has several advantages in routine laboratory analysis.
Authors:
Angel González; Núria Hierro; Montserrat Poblet; Albert Mas; José Manuel Guillamón
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  254     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-02-02     Completed Date:  2006-03-23     Revised Date:  2007-02-13    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  123-8     Citation Subset:  IM    
Affiliation:
Unitat d'Enologia del Centre deReferència de Tecnología d'Aliments, Dept Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Tarragona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acetobacteraceae / genetics,  isolation & purification*
Colony Count, Microbial
Culture Media
DNA Primers
DNA, Bacterial / analysis,  isolation & purification
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Wine / microbiology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA Primers; 0/DNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections
Erratum In:
FEMS Microbiol Lett. 2007 Feb;267(1):129

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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