Document Detail


Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination-free flow cytometry.
MedLine Citation:
PMID:  21246706     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Circulating tumor cells (CTC) are an important biomarker for several solid cancers. Most of the commercially available systems for enumeration of CTC are based on immunomagnetic enrichment of epithelial cell adhesion molecule (EpCAM/CD326)-expressing CTC before microscopic cell imaging or reverse-transcription PCR (RT-PCR). The aim of this study was to establish a practical method for enumeration of CTC using a novel flow cytometer that has a disposable microfluidic chip, which is designed to realize absolute cross contamination-free measurements and to collect the analyzed cell sample. Although the process of enumeration and labeling of CTC was optimized for this device, the simplified protocol described here could be applied to other flow cytometers. Cultured cancer cells spiked into normal blood were enriched using MACS® EpCAM-MicroBeads following cell labeling with an allophycocyanin (APC)-conjugated EpCAM mAb, instead of by intracellular staining of cytokeratins (CK). The EpCAM double-positive selection/labeling method allows enumeration of intact CTC, maintenance of cellular integrity, and the concomitant performance of a CTC viability test. The combination of the fine-tuned CTC enrichment process and the cytometric multicolor analysis resulted in a linear relationship between the output cell count and the input cell number from zero to hundreds of cells. In particular, a satisfactory signal/noise ratio was obtained by gate-exclusion of leukocyte signals using an anti-CD45 mAb. The entire process had little influence on the viability of the spiked lung cancer cell PC-9. Measured PC-9 and breast cancer MCF-7 cells bearing EpCAM-MicroBeads, APC-conjugated EpCAM mAb, and the DNA staining dye SYTO9 grew normally, demonstrating the potential usefulness of the collected samples for further studies. This intact CTC enumeration and analysis procedure (iCeap) would be of great benefit to clinicians by providing them with rapid stratification of antitumor therapy, and to basic researchers by permitting further molecular and cellular characterization of CTC. © 2011 International Society for Advancement of Cytometry.
Authors:
Masashi Takao; Kazuo Takeda
Related Documents :
3342236 - Role of monocarboxylic acid transport in intracellular ph regulation of isolated proxim...
3604176 - The effects of reinflation on the survival of time-expired stored rabbit erythrocytes i...
8923756 - Use of primary cell cultures and intact isolated glandular epithelia for x-ray microana...
3036846 - Characterization of the na+/h+ exchanger during maturation of hl-60 cells induced by di...
7585726 - Cytotoxic and transforming effects of some iron-containing minerals in syrian hamster e...
22434446 - Design, engineering, and construction of photosynthetic microbial cell factories for re...
Publication Detail:
Type:  Journal Article     Date:  2011-01-18
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  79     ISSN:  1552-4930     ISO Abbreviation:  Cytometry A     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  107-17     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 International Society for Advancement of Cytometry.
Affiliation:
Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan. mtakao@idac.tohoku.ac.jp.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Veno-occlusive disease in pediatric patients affected by Wilms tumor.
Next Document:  Cellular and muscular growth patterns during sipunculan development.