Document Detail

Enumeration of absolute cell counts using immunophenotypic techniques.
MedLine Citation:
PMID:  18770719     Owner:  NLM     Status:  MEDLINE    
Absolute counting of cells or cell subsets has a number of significant clinical applications: monitoring the disease status of HIV-infected patients, enumerating residual white blood cells in leukoreduced blood products, and assessing immunodeficiency in a variety of situations. The single-platform method (flow cytometry alone) has emerged as the method of choice for absolute cell enumeration. This technology counts only the cells of interest in a precisely determined blood volume. Exact cell identification is accomplished by a logical electronic gating algorithm capable of identifying lineage-specific immunofluorescent markers. Exclusion of unwanted cells is automatic. This extensive and detailed unit presents protocols for both volumetric and flow-rate determination of residual white blood cells and of leukocyte subsets.
F Mandy; B Brando
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Chapter 6     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-09-04     Completed Date:  2008-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 6.8     Citation Subset:  IM    
Health Canada, Ottawa, Canada.
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MeSH Terms
Antigens, CD34 / biosynthesis
Antigens, CD4 / biosynthesis
Antigens, CD8 / biosynthesis
Cell Count / methods*
Cell Lineage
Cell Separation
Flow Cytometry
HIV Infections / blood
Immunophenotyping / methods*
Leukocytes / cytology*
Reg. No./Substance:
0/Antigens, CD34; 0/Antigens, CD4; 0/Antigens, CD8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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