Document Detail

Enumeration of Salmonella in table eggs, pasteurized egg products and egg containing dishes using quantitative real-time PCR.
MedLine Citation:
PMID:  24362433     Owner:  NLM     Status:  Publisher    
Salmonellae are a major cause of food-borne outbreaks in Europe with eggs and egg products identified as major sources. Due to the low levels of Salmonella in eggs and egg products, direct quantification is difficult. In the present study enrichment quantitative real-time PCR (qPCR) was employed for enumeration of Salmonella in different matrices: table eggs, pasteurized egg products and egg containing dishes. Salmonella enterica serovar Enteritidis (S. Enteritidis) and S. Tennessee were used to artificially contaminate these matrices. Results showed a linear regression between the numbers of Salmonella and the quantification cycle (Cq) values for all matrices used with the exception of pasteurized egg white. Standard curves were constructed using both stationary phase cells and heat stressed cells with similar results. Finally the method was used to evaluate the fate of Salmonella in two egg containing dishes: long egg and tiramisu at abused refrigeration temperatures, and results indicated growth of bacteria over a week period. In conclusion enrichment qPCR was shown to be reliable for enumeration of Salmonella in different egg products.
Dziuginta Jakociune; Frédérique Pasquali; Cristiana Soares da Silva; Charlotta Löfström; Jeffrey Hoorfar; Günter Klein; Gerardo Manfreda; John Elmerdahl Olsen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-20
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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