Document Detail


Entry and intracellular localization of Legionella dumoffii in Vero cells.
MedLine Citation:
PMID:  9480789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Organisms of some Legionella species are known to internalize and multiply within epithelial cell lines. During the study on interaction between Legionella spp. and Vero cells, we found that L. dumoffii Tex-KL (ATCC 33343) can enter into Vero cells approximately four to 20 times more often than five other strains of four species of legionella. The mode of entry between L. dumoffii Tex-KL and L. pneumophila Philadelphia-1 was compared and studied by treating Vero cells with reagents which inhibit phagocytosis and endocytosis. Monodansylcadaverine, cytochalasin D and nocodazol were used as inhibitors of receptor-mediated endocytosis, microfilament-dependent phagocytosis and polymerization of microtubules, respectively. The uptake of L. dumoffii Tex-KL required receptor-mediated endocytosis by Vero cells, while the uptake of L. pneumophila Philadelphia-1 used mainly microfilament-dependent phagocytosis. Polymerization of microtubules was necessary for Vero cells for the uptake of both strains of legionella. An electron microscopic examination revealed that some organisms of the L. dumoffii strain Tex-KL escaped from endosomal vacuoles into cytoplasm in the early stage of infection, and proliferated in the cytoplasm. At that period, most of the bacteria were surrounded by rough endoplasmic reticula. In contrast, L. pneumophila Philadelphia-1 proliferated only within ribosome-lined endosome. We suggest that L. dumoffii Tex-KL internalize and proliferate in Vero cells in a different way to L. pneumophila Philadelphia-1, and that there is a variety of the mode of interaction between Legionella spp. and epithelial cells.
Authors:
K Maruta; M Ogawa; H Miyamoto; K Izu; S I Yoshida
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbial pathogenesis     Volume:  24     ISSN:  0882-4010     ISO Abbreviation:  Microb. Pathog.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-04-09     Completed Date:  1998-04-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  65-73     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press Limited.
Affiliation:
Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, 807, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cadaverine / analogs & derivatives,  pharmacology
Cercopithecus aethiops
Cytochalasin D / pharmacology
Cytoplasm / microbiology*
Endocytosis / drug effects
Endosomes / microbiology*
Hexoses / pharmacology
Legionella / growth & development,  pathogenicity*
Legionella pneumophila / growth & development,  pathogenicity*,  ultrastructure
Macrophages, Peritoneal / microbiology
Mice
Microfilaments / physiology
Microscopy, Electron
Microtubules / physiology
Nocodazole / pharmacology
Phagocytosis / drug effects
Receptors, Cell Surface / physiology
Vero Cells
Chemical
Reg. No./Substance:
0/Hexoses; 0/Receptors, Cell Surface; 10121-91-2/monodansylcadaverine; 22144-77-0/Cytochalasin D; 31430-18-9/Nocodazole; 462-94-2/Cadaverine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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