Document Detail

Entry into cells and selective degradation of tRNAs by a cytotoxic member of the RNase A family.
MedLine Citation:
PMID:  11839736     Owner:  NLM     Status:  MEDLINE    
Onconase (P-30 protein), an enzyme in the ribonuclease A superfamily, exerts cytostatic, cytotoxic, and antiviral activity when added to the medium of growing mammalian cells. We find that onconase enters living mammalian cells and selectively cleaves tRNA with no detectable degradation of rRNA. The RNA specificity of onconase in vitro using reticulocyte lysate and purified RNA substrates indicates that proteins associated with rRNA protect the rRNA from the onconase ribonucleolytic action contributing to the cellular tRNA selectivity of onconase. The onconase-mediated tRNA degradation in cells appears to be accompanied by increased levels of tRNA turnover and induction of tRNA synthesis perhaps in response to the selective toxin-induced loss of tRNA. Degradation products of tRNA(3)(Lys), which acts as a primer for HIV-1 replication, were clearly detected in cells infected with HIV-1 and treated with sublethal concentrations of onconase. However, a new synthesis of tRNA(3)(Lys) also seemed to occur in these cells resulting in plateauing of the steady-state levels of this tRNA. We conclude that the degradation of tRNAs may be a primary factor in the cytotoxic activity of onconase.
Shailendra K Saxena; Ravi Sirdeshmukh; Wojciech Ardelt; Stanislaw M Mikulski; Kuslima Shogen; Richard J Youle
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Publication Detail:
Type:  Journal Article     Date:  2002-02-11
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  277     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-22     Completed Date:  2002-05-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15142-6     Citation Subset:  IM    
Biochemistry Section, Surgical Neurology Branch, NINDS, National Institutes of Health, Bethesda, Maryland 20892-1414, USA.
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MeSH Terms
Biological Transport
Electrophoresis, Polyacrylamide Gel
RNA, Transfer / metabolism*
Ribonucleases / metabolism*
Transcriptional Activation
Reg. No./Substance:
9014-25-9/RNA, Transfer; EC 3.1.-/Ribonucleases; EC 3.1.-/ranpirnase

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