Document Detail

Entry of a heparan sulphate-binding HRV8 variant strictly depends on dynamin but not on clathrin, caveolin, and flotillin.
MedLine Citation:
PMID:  21262518     Owner:  NLM     Status:  MEDLINE    
The major group human rhinovirus type 8 can enter cells via heparan sulphate. When internalized into ICAM-1 negative rhabdomyosarcoma (RD) cells, HRV8 accumulated in the cells but caused CPE only after 3 days when used at high MOI. Adaptation by three blind passages alternating between RD and HeLa cells resulted in variant HRV8v with decreased stability at acidic pH allowing for productive infection in the absence of ICAM-1. HRV8v produced CPE at 10 times lower MOI within 1 day. Confocal fluorescence microscopy colocalization and the use of pharmacological and dominant negative inhibitors revealed that viral uptake is clathrin, caveolin, and flotillin independent. However, it is blocked by dynasore, amiloride, and EIPA. Furthermore, HRV8v induced FITC-dextran uptake and colocalized with this fluid phase marker. Except for the complete inhibition by dynasore, the entry pathway of HRV8v via HS is similar to that of HRV14 in RD cells that overexpress ICAM-1.
Abdul Ghafoor Khan; Angela Pickl-Herk; Leszek Gajdzik; Thomas C Marlovits; Renate Fuchs; Dieter Blaas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-22
Journal Detail:
Title:  Virology     Volume:  412     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2011-05-03     Revised Date:  2011-10-04    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  55-67     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Dept. Med. Biochem., Max F. Perutz Laboratories, Vienna Biocenter, Medical University of Vienna, Dr. Bohr Gasse 9/3, Vienna, Austria.
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MeSH Terms
Caveolins / genetics,  metabolism
Cell Line
Clathrin / genetics,  metabolism
Cytopathogenic Effect, Viral
Dynamin II / genetics,  metabolism*
Host-Pathogen Interactions*
Intercellular Adhesion Molecule-1 / genetics,  metabolism*
Membrane Proteins / genetics,  metabolism
Rhinovirus / genetics,  physiology*
Serial Passage
Virus Internalization*
Grant Support
P 18693-B09//Austrian Science Fund FWF
Reg. No./Substance:
0/Caveolins; 0/Clathrin; 0/Membrane Proteins; 0/flotillins; 126547-89-5/Intercellular Adhesion Molecule-1; EC II

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