| Entry of a heparan sulphate-binding HRV8 variant strictly depends on dynamin but not on clathrin, caveolin, and flotillin. | |
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MedLine Citation:
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PMID: 21262518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The major group human rhinovirus type 8 can enter cells via heparan sulphate. When internalized into ICAM-1 negative rhabdomyosarcoma (RD) cells, HRV8 accumulated in the cells but caused CPE only after 3 days when used at high MOI. Adaptation by three blind passages alternating between RD and HeLa cells resulted in variant HRV8v with decreased stability at acidic pH allowing for productive infection in the absence of ICAM-1. HRV8v produced CPE at 10 times lower MOI within 1 day. Confocal fluorescence microscopy colocalization and the use of pharmacological and dominant negative inhibitors revealed that viral uptake is clathrin, caveolin, and flotillin independent. However, it is blocked by dynasore, amiloride, and EIPA. Furthermore, HRV8v induced FITC-dextran uptake and colocalized with this fluid phase marker. Except for the complete inhibition by dynasore, the entry pathway of HRV8v via HS is similar to that of HRV14 in RD cells that overexpress ICAM-1. |
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Authors:
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Abdul Ghafoor Khan; Angela Pickl-Herk; Leszek Gajdzik; Thomas C Marlovits; Renate Fuchs; Dieter Blaas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-22 |
Journal Detail:
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Title: Virology Volume: 412 ISSN: 1096-0341 ISO Abbreviation: Virology Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-14 Completed Date: 2011-05-03 Revised Date: 2011-10-04 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 55-67 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Dept. Med. Biochem., Max F. Perutz Laboratories, Vienna Biocenter, Medical University of Vienna, Dr. Bohr Gasse 9/3, Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Caveolins
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genetics,
metabolism Cell Line Clathrin / genetics, metabolism Cytopathogenic Effect, Viral Dynamin II / genetics, metabolism* Host-Pathogen Interactions* Humans Intercellular Adhesion Molecule-1 / genetics, metabolism* Membrane Proteins / genetics, metabolism Rhinovirus / genetics, physiology* Serial Passage Virus Internalization* |
| Grant Support | |
ID/Acronym/Agency:
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P 18693-B09//Austrian Science Fund FWF |
| Chemical | |
Reg. No./Substance:
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0/Caveolins; 0/Clathrin; 0/Membrane Proteins; 0/flotillins; 126547-89-5/Intercellular Adhesion Molecule-1; EC 3.6.5.5/Dynamin II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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