Document Detail

Entrapment of Saccharomyces cerevisiae and 3T3 fibroblast cells into blue light cured hydrogels.
MedLine Citation:
PMID:  22678829     Owner:  NLM     Status:  Publisher    
Hydrogels, containing yeast cells or fibroblast cells, were fabricated using blue light-induced polymerization technique. The cell-loaded prepolymer formulation was comprised of poly(ethyleneglycol) diacrylate (more than or equal to 50% v/v), 0.5 wt % Eosin Y and 0.5 wt % triethanolamine as the base oligomer, photo-initiator, and co-initiator, respectively. The two model cell lines, Saccharomyces cerevisiae and NIH 3T3 fibroblasts maintained high viability pre- and post-processing. Several bioassays have demonstrated the unaffected intracellular and extracellular activities of the cells entrapped within the hydrogels. Scanning electron microscopy confirmed the proliferation of S. cerevisiae cells that were entrapped and cultivated for 48 h in growth media, which validated the favorable microenvironment and nutrient transport in these gels. Upon entrapment, fibroblast cells remain viable upto 12 h, however they failed to attach within the crosslinked network, thus no further proliferation was observed. The tunable properties of this hydrogel system project it as a useful matrix for specialized biohybrids. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.
Swati Mishra; Frank J Scarano; Paul Calvert
Related Documents :
15799949 - Mitochondria as integrators of information in an early-evolving animal: insights from a...
15050409 - The effects of ethidium bromide induced loss of mitochondrial dna on mitochondrial phen...
21091789 - Cell-density-dependent changes in mitochondrial membrane potential and reactive oxygen ...
20837069 - What makes the mitochondria a killer? can we condition them to be less destructive?
16624679 - Neurodegeneration induced by complex i inhibition in a cellular model of familial amyot...
20665559 - Injury and differentiation following inhibition of mitochondrial respiratory chain comp...
18239849 - Development of mucilage cells of araucaria angustifolia (araucariaceae).
12089019 - Expression of a fungal hydrophobin in the saccharomyces cerevisiae cell wall: effect on...
23466409 - Development of a scanning electrochemical microscopy-based micropipette and its applica...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-7
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Bioengineering, University of Massachusetts Dartmouth, N. Dartmouth, Massachusetts 02747.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  CXCR6: The Role of Environment in Tumor Progression. Challenges for Therapy.
Next Document:  Acceptability of Intanza® 15 ?g intradermal influenza vaccine in Belgium during the 2010-2011 influ...