Document Detail

Entrapment of Saccharomyces cerevisiae and 3T3 fibroblast cells into blue light cured hydrogels.
MedLine Citation:
PMID:  22678829     Owner:  NLM     Status:  Publisher    
Hydrogels, containing yeast cells or fibroblast cells, were fabricated using blue light-induced polymerization technique. The cell-loaded prepolymer formulation was comprised of poly(ethyleneglycol) diacrylate (more than or equal to 50% v/v), 0.5 wt % Eosin Y and 0.5 wt % triethanolamine as the base oligomer, photo-initiator, and co-initiator, respectively. The two model cell lines, Saccharomyces cerevisiae and NIH 3T3 fibroblasts maintained high viability pre- and post-processing. Several bioassays have demonstrated the unaffected intracellular and extracellular activities of the cells entrapped within the hydrogels. Scanning electron microscopy confirmed the proliferation of S. cerevisiae cells that were entrapped and cultivated for 48 h in growth media, which validated the favorable microenvironment and nutrient transport in these gels. Upon entrapment, fibroblast cells remain viable upto 12 h, however they failed to attach within the crosslinked network, thus no further proliferation was observed. The tunable properties of this hydrogel system project it as a useful matrix for specialized biohybrids. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.
Swati Mishra; Frank J Scarano; Paul Calvert
Related Documents :
17343909 - Bhk cells transfected with ncx3 are more resistant to hypoxia followed by reoxygenation...
19832699 - Mitochondrial targeting of cyclosporin a enables selective inhibition of cyclophilin-d ...
21091789 - Cell-density-dependent changes in mitochondrial membrane potential and reactive oxygen ...
3995499 - Effects of succinylacetone on growth and respiration of l1210 leukemia cells.
19428369 - Mitochondrial mutant cells are hypersensitive to ionizing radiation, phleomycin and mit...
16336229 - Molecular characterization of mitocalcin, a novel mitochondrial ca2+-binding protein wi...
18276199 - Gene delivery by electroporation after dielectrophoretic positioning of cells in a non-...
20570939 - Influence of fat-hippo and notch signaling on the proliferation and differentiation of ...
3736019 - Epithelial-interstitial cell interactions in fetal rat lung development accelerated by ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-7
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  -     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Department of Bioengineering, University of Massachusetts Dartmouth, N. Dartmouth, Massachusetts 02747.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  CXCR6: The Role of Environment in Tumor Progression. Challenges for Therapy.
Next Document:  Acceptability of Intanza® 15 ?g intradermal influenza vaccine in Belgium during the 2010-2011 influ...