| Enterotoxigenic Klebsiella pneumoniae in acute childhood diarrhoea. | |
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MedLine Citation:
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PMID: 1778616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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K. pneumoniae strains recovered as pure or predominant isolate from stool specimens of 50 children below three years of age, presenting with acute watery diarrhoea, were studied for heat-labile enterotoxin production. Twenty three (46%) of the 50 crude toxin concentrates showed positivity in rabbit ileal loops and skin permeability tests. Antigenically 17 (34%) and 20 (40%) of the toxin extracts reacted with immuno-purified anti H-LT antibody in latex particle agglutination and immuno-dot blot assays respectively. Polyacrylamide-gel electrophoresis, Western-blotting and enzyme-immunoassay revealed heat-labile enterotoxin and its subunits in 19 (68%) of 28 extracts tested. In 5 of 10 strains tested the toxigenicity could be transferred to recipient Escherichia coli J-35 in plasmid transfer experiments. Klebsiella induced enterotoxigenic diarrhoea and produced a heat-labile toxin which seems to be biologically, antigenically and possibly genetically related to the heat-labile toxin of the cholera-coli family. |
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Authors:
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D Panigrahi; P Roy; A Chakrabarti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Indian journal of medical research Volume: 93 ISSN: 0971-5916 ISO Abbreviation: Indian J. Med. Res. Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1992-03-11 Completed Date: 1992-03-11 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0374701 Medline TA: Indian J Med Res Country: INDIA |
Other Details:
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Languages: eng Pagination: 293-6 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, Postgraduate Institute of Medical Education & Research, Chandigarh. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Child, Preschool Diarrhea / microbiology* Diarrhea, Infantile / microbiology* Enterotoxins / biosynthesis* Feces / microbiology Humans Infant Klebsiella Infections / microbiology* Klebsiella pneumoniae / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enterotoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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