| Enterostatin efflux in cat intestinal lymph: relation to lymph flow, hyaluronan, and fat absorption. | |
| | |
MedLine Citation:
|
PMID: 8897893 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The question addressed in this study was whether enterostatin, the pancreatic procolipase activation peptide, modulates intestinal hyaluronan turnover via lymph. In anesthetized cats, segments of ileum were surgically isolated from the proximal and distal gut, the draining lymphatic was cannulated, and the segment was autoperfused in situ. In several groups, concentrations of immunoreactive enterostatin in lymph were compared with that in plasma at baseline and elevated lymph flow and in the absence and presence of fat absorption. The baseline ratio of lymph enterostatin to that in plasma (L/P) in the absence of fat absorption was 1.44 +/- 0.29 compared with 4.93 +/- 0.42 after cream feeding (P < 0.05). In a separate group, when the intestinal lumen was perfused for 2 h with a mixture of oleic acid and taurocholate, enterostatin L/P doubled compared with baseline. At high lymph flows, enterostatin concentrations fell in all groups, resulting in an L/P of 0.47 +/- 0.09 (P < 0.05) in the absence of fat absorption, 0.77 +/- 0.35 after oleic acid, and 1.26 +/- 0.13 in the cream-fed group. These changes correlate with the pattern of hyaluronan efflux from the ileum into lymph after fat absorption [R.K. Reed, M.I Townsley, V.H. Pitts, T.C. Laurent, and A.E. Taylor. Am. J. Physiol, 263 (Gastrointest. Liver Physiol. 26): G6-G11, 1992] However, in separate groups when enterostatin was introduced into ileum, either as a close intra-arterial bolus or via the intestinal lumen, there were no resultant changes in efflux of hyaluronan from the intestine into lymph. In conclusion, despite the fact that delivery of pancreatic exocrine secretions to the ileal lumen was blocked in this model, enterostatin concentration in lymph increased after fat absorption. Nonetheless, it seems clear that enterostatin does not modify intestinal hyaluronan turnover. |
| | |
Authors:
|
M I Townsley; C Erlanson-Albertsson; A Ohlsson; C Rippe; R K Reed |
Related Documents
:
|
17349083 - Effects of dietary soyabean meal, inulin and oxytetracycline on intestinal microbiota a... 16582093 - Measuring splanchnic amino acid metabolism in vivo using stable isotopic tracers. 9772143 - The water-soluble extract of chicory influences serum and liver lipid concentrations, c... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Oct |
Date Detail:
|
Created Date: 1996-12-16 Completed Date: 1996-12-16 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: G714-21 Citation Subset: IM |
Affiliation:
|
Department of Physiology, University of South Alabama, Mobile 36688, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cats Colipases / pharmacology* Enzyme Precursors Hyaluronic Acid / metabolism* Intestinal Absorption Lipid Metabolism* Lymph / physiology* Lymphatic System / physiology Protein Precursors / pharmacology* Rheology |
| Grant Support | |
ID/Acronym/Agency:
|
HL-39045/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Colipases; 0/Enzyme Precursors; 0/Protein Precursors; 72994-53-7/procolipase; 9004-61-9/Hyaluronic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of sucrase and lactase in Caco-2 cells: relationship to nuclear factors SIF-1 and NF-LPH-...
Next Document: Cell cycle protein suppression and p21 induction in differentiating Caco-2 cells.