| Enterohepatic physiology of 1,25-dihydroxyvitamin D3. | |
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MedLine Citation:
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PMID: 7356679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After intravenous administration of radiolabeled 1,25-dihydroxyvitamin D3 to rats, approximately 25% of the administered radioactivity appeared in the bile within 24 h. Instillation of the biliary radioactivity into the duodena of other rats was followed by recovery of 15% of the radioactivity in newly secreted bile within 24 h. The process by which products of 1,25-dihydroxyvitamin D3 were excreted in bile was not saturable in the dose range tested (0.275-650 ng). The metabolites of 1,25-dihydroxyvitamin D3 present in bile were found to be much more polar than 1,25-dihydroxyvitamin D3 and were resolved into three fractions on high performance liquid chromatography. 60% of the radioactivity present in bile was retained selectively by DEAE-cellulose; the radioactive material could be eluted from the gel at a low pH or at high salt concentrations. When bile containing the radiolabeled metabolites was incubated at 37 degrees C and pH 5 with beta-glucuronidase, there was an increase in the amount of radioactivity comigrating with 1,25-dihydroxyvitamin D3. Treatment of the products of radiolabeled 1,25-dihydroxyvitamin D3 in bile with diazomethane, an agent which converts acids into methyl esters, transformed one of the metabolites into a less polar compound. These results demonstrate that there is a quantitatively important enterophepatic circulation of the products of 1,25-dihydroxyvitamin D3 in the rat. |
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Authors:
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R Kumar; S Nagubandi; V R Mattox; J M Londowski |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 65 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1980 Feb |
Date Detail:
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Created Date: 1980-03-24 Completed Date: 1980-03-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 277-84 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / drug effects, metabolism* Dihydroxycholecalciferols / metabolism* Duodenum / metabolism Enterohepatic Circulation* Glucuronidase / pharmacology Hydroxycholecalciferols / metabolism* Male Rats Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Dihydroxycholecalciferols; 0/Hydroxycholecalciferols; EC 3.2.1.31/Glucuronidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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