Document Detail

Enteroendocrine cells in terminal ileal Crohn's disease.
MedLine Citation:
PMID:  22398079     Owner:  NLM     Status:  Publisher    
BACKGROUND AND AIMS: Enteroendocrine cells sense gut luminal contents, and orchestrate digestive physiology whilst contributing to mucosal homeostasis and innate immunity. The terminal ileum is the key site of EEC expression but detailed assessment of their subtypes, lineage transcription factors and expression products has not been undertaken in terminal ileal Crohn's disease. Recent Crohn's disease gene wide association studies have linked the neuroendocrine transcription factor Phox2b; while autoantibodies to an enteroendocrine protein, ubiquitination protein 4a, have been identified as a disease behaviour biomarker. METHODS: Terminal ileal tissue from small or large bowel Crohn's disease and normal controls was analysed for enteroendocrine marker expression by immunohistochemistry and quantitative polymerase chain reaction. Inflammation was graded by endoscopic, clinical, histological and biochemical scoring. RESULTS: In small bowel disease, glucagon-like peptide 1 and chromogranin A cells were increased 2.5-fold (p=0.049) and 2-fold (p=0.031) respectively. Polypeptide YY cells were unchanged. Ileal enteroendocrine cell expression was unaffected in the presence of Crohn's colitis. Phox2b was co-localised to enteroendocrine cells and showed a 1.5-fold increase in ileal disease. Significant mRNA increases were noted for chromogranin A (3.3-fold; p=0.009), glucagon-like peptide 1 (3.1-fold; p=0.007) and ubiquitination protein 4a (2.2-fold; p=0.02). Neurogenin 3, an enteroendocrine transcription factor showed ~2 fold-upregulation (p=0.048). CONCLUSIONS: Enhanced enteroendocrine cell activity is present in small bowel disease, and observed in restricted cell lineages. This may impact on the epithelial immune response, cellular homeostasis and nutrient handling and influence appetite via increased satiety signalling in the gut-brain axis.
Gordon W Moran; Joanne Pennock; John T McLaughlin
Related Documents :
19627499 - Spxa1, a novel transcriptional regulator involved in x-state (competence) development i...
10775269 - Regulation of plastid rdna transcription by interaction of cdf2 with two different rna ...
9032299 - Constitutive expression in gal7 mutants of kluyveromyces lactis is due to internal prod...
7504669 - Differential decay of rna of the cfa/i fimbrial operon and control of relative gene exp...
3034429 - Lac repressor can regulate expression from a hybrid sv40 early promoter containing a la...
3297789 - Studies with the escherichia coli galactose operon regulatory region carrying a point m...
15744669 - Developmental expression of pod 1 in xenopus laevis.
18787619 - Expression of 14-3-3sigma, p16 and p53 proteins in anal squamous intraepithelial neopla...
8352739 - Cloning of the gene for glutamate decarboxylase and its expression during conidiation i...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-3
Journal Detail:
Title:  Journal of Crohn's & colitis     Volume:  -     ISSN:  1876-4479     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318676     Medline TA:  J Crohns Colitis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 European Crohn's and Colitis Organisation. Published by Elsevier B.V. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Anti-TNF therapy induced immune neutropenia in Crohns disease- report of 2 cases and review of liter...
Next Document:  Temporal trends in colon neoplasms in patients with primary sclerosing cholangitis and ulcerative co...