Document Detail

Enterocytozoon bieneusi (microsporidia) in clinical samples from immunocompetent individuals in Tenerife, Canary Islands, Spain.
MedLine Citation:
PMID:  16111728     Owner:  NLM     Status:  MEDLINE    
Microsporidia are newly emerging pathogens of humans and animals, with Enterocytozoon bieneusi being the most common causal agent in human microsporidiosis. To determine the presence of E. bieneusi, 273 clinical samples (40 urine, 156 stools, 37 sputum, 9 bronchial aspirates, 5 bronchial washes and 26 pleural fluids) from immunocompetent patients, mainly suffering diarrhoea or pneumonia, in Tenerife, Canary Islands, Spain were analysed using light microscopy after staining with Weber's chromotrope and by PCR/hybridisation with a specific probe designed to increase the sensitivity of the identification. In this study, detection of E. bieneusi after PCR/hybridisation is reported in 18 (11.54%) of 156 stool samples, 1 (2.5%) of 40 urine samples and 6 (16.22%) of 37 sputum samples. To our knowledge, these are the first reports of E. bieneusi in this subtropical region, showing the increased importance of these parasites as emerging pathogens worldwide.
Néstor Abreu-Acosta; Jacob Lorenzo-Morales; Yaiza Leal-Guio; Nieves Coronado-Alvarez; Pilar Foronda; Julia Alcoba-Florez; Fernando Izquierdo; Ninive Batista-Díaz; Carmen Del Aguila; Basilio Valladares
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transactions of the Royal Society of Tropical Medicine and Hygiene     Volume:  99     ISSN:  0035-9203     ISO Abbreviation:  Trans. R. Soc. Trop. Med. Hyg.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-09-05     Completed Date:  2006-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506129     Medline TA:  Trans R Soc Trop Med Hyg     Country:  England    
Other Details:
Languages:  eng     Pagination:  848-55     Citation Subset:  IM    
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Avda. Astrofísico Fco. Sánchez, S/N 38203, La Laguna, Canary Islands, Spain.
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MeSH Terms
Aged, 80 and over
Atlantic Islands / epidemiology
Child, Preschool
Indian Ocean Islands / epidemiology
Microsporidia / isolation & purification*
Microsporidiosis / diagnosis*,  epidemiology
Middle Aged
Sensitivity and Specificity
Spain / epidemiology

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