| Enteric glial cells are major contributors to formation of cyclic AMP in myenteric plexus cultures from adult guinea-pig small intestine. | |
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MedLine Citation:
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PMID: 8264948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultures derived from ganglia isolated from the small intestine of adult guinea-pigs were used to determine relative contribution of neurons and glial cells to stimulation of cAMP formation by forskolin in myenteric ganglia. In untreated cultures (8-12 days), the ratio of glial cells to neurons was 5-fold higher than the ratio in intact myenteric plexus preparations. Treatment with cytosine arabinoside virtually eliminated the glia by the 12th day. Microelectrode recording of excitatory responses to forskolin in AH/Type 2 neurons confirmed the viability of cultured neurons in cytosine arabinoside. Forskolin elevated the cAMP content of cultures and cytosine arabinoside reduced this effect by 80-90%. This suggests that enteric glial cells are the major contributors to cAMP formation in the cultures and that glial cells contribute significantly to elevation of cAMP levels seen in intact myenteric ganglia. |
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Authors:
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F L Christofi; M Hanani; N Maudlej; J D Wood |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuroscience letters Volume: 159 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1994-01-25 Completed Date: 1994-01-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 107-10 Citation Subset: IM |
Affiliation:
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Department of Physiology, Ohio State University, College of Medicine, Columbus 43210-1218. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cyclic AMP / biosynthesis* Cytarabine / pharmacology Enteric Nervous System / cytology, metabolism* Forskolin / pharmacology Ganglia / cytology, metabolism Guinea Pigs Intestine, Small / cytology, innervation, metabolism* Microelectrodes Microscopy, Fluorescence Mitosis / drug effects Myenteric Plexus / cytology, metabolism* Neuroglia / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK39937/DK/NIDDK NIH HHS; R29 DK44179-02/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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147-94-4/Cytarabine; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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