| Enteral supplementation enriched with glutamine, fiber, and oligosaccharide prevents gut translocation in a bacterial overgrowth model. | |
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MedLine Citation:
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PMID: 19131813 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Normal gut flora plays an important role in the intestinal mucosal barrier function under various critical conditions. The flora may alter after severe insults, such as trauma and shock. Enteral nutrition should preserve the gut environment; however, full support is usually difficult for severely ill patients because of impaired gastrointestinal motility. Currently, we have commercial enteral supplementation product enriched with glutamine, dietary fiber, and oligosaccharide (GFO) in Japan. This study examines the hypothesis that the enteral supplementation ameliorates gut injury induced by a bacterial overgrowth model, even in small volumes and quantities. MATERIALS: Balb/c mice received antibiotics (4 mg/mL of streptomycin) in their drinking water for 4 days to kill the normal gut flora after which they were orally inoculated with a streptomycin-resistant strain of Escherichia coli, known as E. coli C-25. The mice that were administered bacterial monoassociation received 0.5 mL of GFO twice daily (GFO group) or 10% of glucose solution (GLU group). Unsupplemented drinking water was used for control animals (control) whose gut flora was normal. The mice were killed and their mesenteric lymph nodes complex was harvested and processed to test gut bacterial translocation. The cecal population levels of bacteria and ileum histology were also examined. RESULTS: The incidence and magnitude of gut translocation to the lymph nodes complex in the GLU group were significantly higher than those in the control (p < 0.01). Treatment with GFO prevented the gut translocation although animals in the GFO group had same level of the cecal bacterial population. Histologic findings in the ileum were not different between the GLU and GFO. CONCLUSION: GFOs supplement prevented gut translocation for bacterial overgrowth even in small volumes and quantities. The intestinal histologic findings could not explain the protective mechanisms of GFO. Further studies may be needed to elucidate the benefit of the partial enteral nutrition. |
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Authors:
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Hikohiro Azuma; Shiro Mishima; Jun Oda; Hiroshi Homma; Hirokazu Sasaki; Masaki Hisamura; Shoichi Ohta; Tetsuo Yukioka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of trauma Volume: 66 ISSN: 1529-8809 ISO Abbreviation: J Trauma Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-09 Completed Date: 2009-02-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376373 Medline TA: J Trauma Country: United States |
Other Details:
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Languages: eng Pagination: 110-4 Citation Subset: AIM; IM |
Affiliation:
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Department of Emergency and Critical Care Medicine, Tokyo Medical University, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Bacterial Translocation / drug effects* Chi-Square Distribution Dietary Fiber / administration & dosage* Enteral Nutrition / methods* Glutamine / administration & dosage* Intestines / microbiology* Lymph Nodes / microbiology Male Mice Mice, Inbred BALB C Oligosaccharides / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides; 56-85-9/Glutamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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