| Enteral exclusion increases MAP kinase activation and cytokine production in a model of gallstone pancreatitis. | |
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MedLine Citation:
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PMID: 18235211 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We have previously demonstrated that enteral exclusion augments pancreatic p38 mitogen-activated protein (MAP) kinase activation and tumor necrosis factor-alpha (TNF-alpha) production after bile-pancreatic duct ligation in rats. METHODS: In the present study, we evaluated c-Jun NH(2)-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) activation, and cytokine production, in pancreata of duct-ligated rats with and without duodenal bile-pancreatic juice replacement from a donor rat. We hypothesized that enteral exclusion of bile-pancreatic juice activates stress kinases and induces cytokine production in ligation-induced acute pancreatitis. RESULTS: Increased JNK and ERK activation after ligation are inhibited by bile-pancreatic juice replacement. Increases in pancreatic production of IL-1beta and IL-12 after ligation are significantly subdued by replacement. In additional in vitro studies, we show that cholecystokinin- or TNF-alpha-stimulated nuclear transcription factor kappa-B activation in AR42J cells is inhibited by dominant negative ERK2. CONCLUSIONS: Our novel findings using our Donor Rat Model indicate that bile-pancreatic juice exclusion induces MAP kinase activation and exacerbates cell stress and inflammation in this experimental model of gallstone pancreatitis. and IAP. |
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Authors:
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Isaac Samuel; Linda Tephly; Deborah E Williard; A Brent Carter |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-01-31 |
Journal Detail:
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Title: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] Volume: 8 ISSN: 1424-3911 ISO Abbreviation: Pancreatology Publication Date: 2008 |
Date Detail:
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Created Date: 2008-04-24 Completed Date: 2008-07-31 Revised Date: 2011-10-04 |
Medline Journal Info:
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Nlm Unique ID: 100966936 Medline TA: Pancreatology Country: Switzerland |
Other Details:
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Languages: eng Pagination: 6-14 Citation Subset: IM |
Copyright Information:
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Copyright 2008 S. Karger AG, Basel. |
Affiliation:
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Department of Surgery, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA. isaac-samuel@uiowa.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile / metabolism Cell Line, Tumor Cytokines / genetics, metabolism* Disease Models, Animal Enzyme Activation Gallstones / complications* Gene Expression Regulation Inflammation Male Mitogen-Activated Protein Kinases / genetics, metabolism* Pancreas / metabolism, pathology Pancreatic Juice / metabolism Pancreatitis / blood, complications* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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DK062805/DK/NIDDK NIH HHS; R01 ES014871-05/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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