Document Detail


Enteral diets enriched with medium-chain triglycerides and N-3 fatty acids prevent chemically induced experimental colitis in rats.
MedLine Citation:
PMID:  20970751     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The specific purpose of this study was to evaluate the significant effects of medium-chain triglycerides (MCTs) and N-3 fatty acids on chemically induced experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS) in rats. Male Wistar rats were fed liquid diets enriched with N-6 fatty acid (control diets), N-3 fatty acid (MCT- diets), and N-3 fatty acid and MCT (MCT+ diets) for 2 weeks and then were given an intracolonic injection of TNBS. Serum and tissue samples were collected 5 days after ethanol or TNBS enema. The severity of colitis was evaluated pathologically, and tissue myeloperoxidase activity was measured in colonic tissues. Furthermore, protein levels for inflammatory cytokines and a chemokine were assessed by an enzyme-linked immunosorbent assay in colonic tissues. Induction of proinflammatory cytokines tumor necrosis factor-α and interleukin-1β in the colon by TNBS enema was markedly attenuated by the MCT+ diet among the 3 diets studied. Furthermore, the induction of chemokines macrophage inflammatory protein-2 and monocyte chemotactic protein-1 also was blunted significantly in animals fed the MCT+ diets. As a result, MPO activities in the colonic tissue also were blunted significantly in animals fed the MCT+ diets compared with those fed the control diets or the MCT- diets. Furthermore, the MCT+ diet improved chemically induced colitis significantly among the 3 diets studied. Diets enriched with both MCTs and N-3 fatty acids may be effective for the therapy of inflammatory bowel disease as antiinflammatory immunomodulating nutrients.
Authors:
Hiroshi Kono; Hideki Fujii; Masahito Ogiku; Masato Tsuchiya; Kenichi Ishii; Michio Hara
Publication Detail:
Type:  Journal Article     Date:  2010-08-24
Journal Detail:
Title:  Translational research : the journal of laboratory and clinical medicine     Volume:  156     ISSN:  1878-1810     ISO Abbreviation:  Transl Res     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-25     Completed Date:  2010-11-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101280339     Medline TA:  Transl Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  282-91     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2010 Mosby, Inc. All rights reserved.
Affiliation:
First Department of Surgery, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan. hkouno@yamanashi.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animals
Body Weight / drug effects
Chemokines / metabolism
Colitis / chemically induced,  pathology,  prevention & control*
Colon / drug effects,  enzymology,  pathology
Disease Models, Animal
Endotoxemia / blood,  drug therapy,  etiology
Endotoxins / blood
Enema
Enteral Nutrition / methods*
Fatty Acids, Omega-3 / administration & dosage*
Male
Peroxidase / metabolism
Rats
Rats, Wistar
Triglycerides / administration & dosage*
Trinitrobenzenesulfonic Acid / toxicity
Chemical
Reg. No./Substance:
0/Chemokines; 0/Endotoxins; 0/Fatty Acids, Omega-3; 0/Triglycerides; 2508-19-2/Trinitrobenzenesulfonic Acid; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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