| Enteral tube feeding alters the oral indigenous microbiota in elderly adults. | |
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MedLine Citation:
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PMID: 21821752 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Enteral tube feeding is widely used to maintain nutrition for elderly adults with eating difficulties, but its long-term use alters the environment of the oral ecosystem. This study characterized the tongue microbiota of tube-fed elderly adults by analyzing the 16S rRNA gene. The terminal restriction fragment length polymorphism (T-RFLP) profiles of 44 tube-fed subjects were compared with those of 54 subjects fed orally (average age, 86.4 ± 6.9 years). Bar-coded pyrosequencing data were also obtained for a subset of the subjects from each group (15 tube-fed subjects and 16 subjects fed orally). The T-RFLP profiles demonstrated that the microbiota of the tube-fed subjects was distinct from that of the subjects fed orally (permutational multivariate analysis of variance [perMANOVA], P < 0.001). The pyrosequencing data revealed that 22 bacterial genera, including Corynebacterium, Peptostreptococcus, and Fusobacterium, were significantly more predominant in tube-fed subjects, whereas the dominant genera in the subjects fed orally, such as Streptococcus and Veillonella, were present in much lower proportions. Opportunistic pathogens rarely detected in the normal oral microbiota, such as Corynebacterium striatum and Streptococcus agalactiae, were often found in high proportions in tube-fed subjects. The oral indigenous microbiota is disrupted by the use of enteral feeding, allowing health-threatening bacteria to thrive. |
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Authors:
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Toru Takeshita; Masaki Yasui; Mikiko Tomioka; Yoshio Nakano; Yoshihiro Shimazaki; Yoshihisa Yamashita |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-08-05 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-23 Completed Date: 2012-01-19 Revised Date: 2012-04-02 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 6739-45 Citation Subset: IM |
Affiliation:
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Section of Preventive and Public Health Dentistry, Division of Oral Health, Growth and Development, Kyushu University Faculty of Dental Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Bacteria / classification*, isolation & purification* Biodiversity* Cluster Analysis DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics Enteral Nutrition / adverse effects* Humans Phylogeny Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Tongue / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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