Document Detail


Entamoeba histolytica interactions with polarized human intestinal Caco-2 epithelial cells.
MedLine Citation:
PMID:  7927794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To model the initial pathogenic effects of Entamoeba histolytica trophozoites on intestinal epithelial cells, the interactions of E. histolytica HM1-IMSS trophozoites with polarized human intestinal Caco-2 cell monolayers grown on permeabilized filters were examined. Trophozoites, when incubated with the apical surface of the monolayers at 37 degrees C, induced a rapid decrease in transepithelial resistance over 15 to 60 min. The transmonolayer resistance response was not associated with changes in short-circuit current but was associated with an increase in mannitol flux, suggesting that the drop in resistance reflected a nonselective increase in epithelial permeability rather than stimulation of electrogenic ion transport. This response preceded the earliest detection of morphologic disruption of monolayer integrity by light or electron microscopy. Apical injury to the monolayer was detected by ultrastructural studies which revealed a loss of brush border in regions of contact between epithelial cells and amebas and by chromium release assays where a small increase in the apical release of 51Cr from the monolayer (6% over background) was observed. The transmonolayer resistance response was inhibited when the temperature was reduced to 4 degrees C and by addition of cytochalasin D (1 microgram/ml) to the medium at concentrations that did not directly affect transmonolayer resistance. Application of amebic lysates or medium conditioned by coincubation of amebas with Caco-2 monolayers failed to lower transmonolayer resistance, suggesting that this effect was not mediated by soluble amebic cytotoxins. Polarized Caco-2 monolayers grown on permeable filters provide a useful model for studying the initial interactions of E. histolytica trophozoites with intestinal epithelial cells.
Authors:
E Li; W F Stenson; C Kunz-Jenkins; P E Swanson; R Duncan; S L Stanley
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  62     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1994-11-22     Completed Date:  1994-11-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5112-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Cell Adhesion
Cell Differentiation
Cell Line
Cell Membrane Permeability
Cell Polarity
Cytochalasin D / pharmacology
Electrophysiology
Entamoeba histolytica / growth & development*
Epithelial Cells
Epithelium / parasitology
Humans
Intestinal Mucosa / parasitology*
Lactose / pharmacology
Leucine / analogs & derivatives,  pharmacology
Mannitol / metabolism
Permeability
Grant Support
ID/Acronym/Agency:
AI30084-01/AI/NIAID NIH HHS; DK33165/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
22144-77-0/Cytochalasin D; 61-90-5/Leucine; 63-42-3/Lactose; 66701-25-5/E 64; 69-65-8/Mannitol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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