Document Detail


Ensembles of engineered cardiac tissues for physiological and pharmacological study: heart on a chip.
MedLine Citation:
PMID:  22072288     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover slips of single cells, isotropic monolayers, or in isolated muscle strips. In all of these cases, variability of the samples hinders quantitative comparisons among experimental groups. Here, we report the design of a "heart on a chip" that exploits muscular thin film technology--biohybrid constructs of an engineered, anisotropic ventricular myocardium on an elastomeric thin film--to measure contractility, combined with a quantification of action potential propagation, and cytoskeletal architecture in multiple tissues in the same experiment. We report techniques for real-time data collection and analysis during pharmacological intervention. The chip is an efficient means of measuring structure-function relationships in constructs that replicate the hierarchical tissue architectures of laminar cardiac muscle.
Authors:
Anna Grosberg; Patrick W Alford; Megan L McCain; Kevin Kit Parker
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-11-10
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-24     Completed Date:  2012-03-19     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  4165-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemistry
Animals
Cells, Cultured
Dimethylpolysiloxanes / chemistry
Epinephrine / pharmacology
Fluorescent Dyes / chemistry
Microfluidic Analytical Techniques / instrumentation*
Myocytes, Cardiac / cytology,  drug effects,  physiology*
Rats
Rats, Sprague-Dawley
Tissue Engineering*
Grant Support
ID/Acronym/Agency:
1 R01 HL079126/HL/NHLBI NIH HHS; 1 U01 NS073474-01/NS/NINDS NIH HHS; R01 HL079126/HL/NHLBI NIH HHS; U01 HL100408/HL/NHLBI NIH HHS; U01 NS073474/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Dimethylpolysiloxanes; 0/Fluorescent Dyes; 25189-55-3/poly-N-isopropylacrylamide; 63148-62-9/baysilon; YKH834O4BH/Epinephrine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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