| Enrichment and properties of urinary pre-S-phenylmercapturic acid (pre-SPMA). | |
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MedLine Citation:
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PMID: 19762294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In benzene metabolism, pre-S-phenylmercapturic acid (pre-SPMA) is the precursor to S-phenylmercapturic acid (SPMA). Urinary pre-SPMA/SPMA ratios are variable. For the determination of urinary SPMA as a biomarker of exposure to benzene it is essential to completely convert pre-SPMA to SPMA. We developed a procedure for the enrichment and determination of urinary pre-SPMA by LC-MS/MS which allowed us to trace the conversion of pre-SPMA to SPMA. Complete conversion was found upon treatment of urine with HCl (37%) at pH 1.1. Previously reported treatment of urine with concentrated H(2)SO(4) was found to yield SPMA levels higher than after HCl treatment. The origin of that extra SPMA amount is unknown. In conclusion, our findings suggest that pre-treatment of urine with HCl to adjust the pH to 0.5-1 is essential for complete conversion of pre-SPMA to SPMA and should be applied prior to analysis of SPMA in urine. |
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Authors:
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Katharina Sterz; Dominique Köhler; Thomas Schettgen; Gerhard Scherer |
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Publication Detail:
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Type: Journal Article Date: 2009-09-04 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 878 ISSN: 1873-376X ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-21 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 2502-5 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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ABF Analytisch-Biologisches-Forschungslabor GmbH, Goethestr. 20, D-80336 Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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analogs & derivatives*,
chemistry,
urine Biological Markers / chemistry, urine* Chromatography, Liquid / methods Hydrogen-Ion Concentration Tandem Mass Spectrometry / methods |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 4775-80-8/S-phenyl-N-acetylcysteine; 616-91-1/Acetylcysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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