Document Detail

Enrichment and identification of fetal trophoblast cells from first trimester maternal cervical lavage and uterine blood specimens.
MedLine Citation:
PMID:  11597189     Owner:  NLM     Status:  MEDLINE    
First trimester prenatal diagnosis of fetal aneuploidies is an active area of research despite years of disappointing data employing maternal peripheral blood samples. To remedy this situation we have investigated other first trimester maternal specimens attempting to find a consistent fetal cell source. Using our previously established positive enrichment procedure along with a commercially available depletion method, fetal trophoblast cells were identified employing immunocytochemistry using an antibody cocktail or by using mRNA in-situ hybridization employing a cocktail of trophoblast specific probes. Fetal origin of positively identified cells was verified using interphase fluorescent in-situ hybridization (FISH) for X and Y-chromosomes. Artificial model systems were established that indicated yields of trophoblast cells and allowed the enrichment procedure to be optimized for minimal losses from maternal specimens. We demonstrate herein that blood drawn from maternal vessels near the placental implantation site to be the most consistent source of fetal cells from any first trimester maternal specimen described to date. In addition, a high yield of multinucleated syncytiotrophoblast cells was obtained using a cell depletion strategy to enrich the target cells. The safety of the procedure or even the clinical utility of blood drawn from maternal vessels near the placental implantation site is yet to be demonstrated.
P A Schueler; D T Yamanishi; E J Collarini; J Pearson; Y Lee; C A Cain; J Edwards; L Foltz; X Wu; W Mahoney
Related Documents :
21390859 - Flow cytometry.
7321579 - Using the quantimet 720 image analyzing computer to count nucleolated neurones in the h...
25356419 - Intrathecal anti-cd20 efficiently depletes meningeal b cells in cns autoimmunity.
11197679 - Automated counting of mammalian cell colonies.
19923619 - Effect of somatic cell count level on functional longevity in valle del belice dairy sh...
25478319 - Genome-wide redistribution of brd4 binding sites in transformation resistant cells.
19768129 - Müller cell activation, proliferation and migration following laser injury.
3695509 - Mechanisms of growth inhibition by nonsteroidal antioestrogens in human breast cancer c...
10531549 - Quantification of responses from proprioceptive neurons in the limbs of the crab, cance...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Placenta     Volume:  22     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2001 Sep-Oct
Date Detail:
Created Date:  2001-10-12     Completed Date:  2001-12-20     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  688-701     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Harcourt Publishers Ltd.
Chief Technology Office, Roche Diagnostics, Berkeley, CA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Separation*
Cervix Uteri / cytology*
Chromosome Aberrations
In Situ Hybridization
In Situ Hybridization, Fluorescence
Pregnancy Trimester, First
Prenatal Diagnosis
RNA, Messenger / analysis
Trophoblasts / cytology*
Uterus / blood supply*
X Chromosome
Y Chromosome
Reg. No./Substance:
0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The characterization of pregnancy associated plasma protein-E and the identification of an alternati...
Next Document:  Inconsistency of fetal trophoblast cells in first trimester maternal peripheral blood prevents non-i...