Document Detail


Enrichment and detection of fetal erythroid cells from maternal peripheral blood using liquid culture.
MedLine Citation:
PMID:  11180235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolating fetal cells from maternal blood for prenatal genetic analysis is the least invasive method currently being investigated. In order to enrich these fetal erythroid cells we employed a two-phase liquid culture system that supports the growth and differentiation of erythroid progenitor cells. Mononuclear cells were separated from ten maternal blood samples at 8-14(+3) weeks of gestation and cultured in the first phase. After 4-5 days, the non-adherent cells were harvested and recultured with erythropoietin for another 3-4 days. The mean number of total erythroid cells reached approximately 0.77x10(6)/ml with an average purity of 90.5%. Hb F stain disclosed fetal erythroid cells averaging approximately 5.7%, therefore the mean number of fetal erythroid cells isolated was 0.49x10(5)/ml. Female karyotypes were only observed while counting 5-66 metaphases. However, male DNA (SRY or DYZ1) was detected by PCR in 7/10 cases; in four of these cases and in one SRY-negative pregnancy the 46,XY karyotype was found by amniocentesis performed during the second trimester. This liquid culture system provided an enriched source of fetal cells without the need for more complicated separation or sorting procedures.
Authors:
J Y Han; Y H Lee; S D Sin; J I Park; I H Kim; G H Je; G P Rodgers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  21     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-04-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  22-6     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Ltd.
Affiliation:
Department of Clinical Pathology, Dong-A University College of Medicine, 1,3-Ga, Tongdaesin-dong, Seo-gu, Pusan, 602-103, Korea. jyhan@damc.dauhosp.or.kr
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MeSH Terms
Descriptor/Qualifier:
Blood Cells / cytology*
Cell Culture Techniques*
Cell Separation / methods*
DNA / analysis
Erythrocyte Count
Erythroid Precursor Cells / cytology*
Erythropoietin / pharmacology
Female
Fetal Hemoglobin / analysis
Fetus*
Gestational Age
Humans
Karyotyping
Male
Polymerase Chain Reaction
Pregnancy
Y Chromosome
Chemical
Reg. No./Substance:
11096-26-7/Erythropoietin; 9007-49-2/DNA; 9034-63-3/Fetal Hemoglobin

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