Document Detail

Enrichment of conjugated linoleic acid in oats (Avena sativa L.) by microbial isomerization.
MedLine Citation:
PMID:  15030240     Owner:  NLM     Status:  MEDLINE    
A method for microbial isomerization of oat linoleic acid to conjugated linoleic acid (CLA) was developed. The method includes hydrolysis of oat lipids in aqueous flour slurries by the endogenous oat lipase. Then, the flour slurry containing free linoleic acid is utilized as a substrate for the isomerization reaction carried out by resting cells of Propionibacterium freudenreichii ssp. shermanii. The isomerization reaction progressed most effectively when, after the lipid hydrolysis period, the pH of the slightly acidic oat slurry was elevated to 8.0-8.5 and maintained at this range. With slurries containing 5% (w/v) oat flour, the amounts of CLA formed per dry matter were up to 10.1 mg/g corresponding to 102 mg/g lipids or 0.44 mg/mL slurry. Increments in the flour content up to 15% increased the volumetric production of CLA to 0.85 mg/mL. The proportion of the cis-9,trans-11 isomer was 80% of the total CLA formed. CLA could be concentrated into the solid material of the oat slurry by acidification.
Marjatta Vahvaselkä; Pekka Lehtinen; Soili Sippola; Simo Laakso
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  52     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-19     Completed Date:  2004-05-07     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1749-52     Citation Subset:  IM    
Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, P.O. Box 9400, FIN-02015 HUT, Finland.
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MeSH Terms
Avena sativa / chemistry*
Hydrogen-Ion Concentration
Linoleic Acid / metabolism
Linoleic Acids, Conjugated / chemistry*,  metabolism*
Lipase / metabolism
Lipid Metabolism
Propionibacterium / metabolism*
Reg. No./Substance:
0/Linoleic Acids, Conjugated; 2197-37-7/Linoleic Acid; EC

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