Document Detail

Enrichment of collagen plugs with platelets and amniotic fluid cells increases cell proliferation in sealed iatrogenic membrane defects in the foetal rabbit model.
MedLine Citation:
PMID:  18435415     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The purpose of this study was to evaluate cell proliferation in platelet-enriched collagen plugs with and without addition of amniotic fluid-derived heterologous foetal cells to seal an iatrogenic membrane defect in the foetal rabbit model. METHODS: Amniotic fluid cells were harvested from three donor does at 23 days of gestation (term = 32 days) and labelled with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE). In 42 other does, foetal membrane defects were induced by foetoscopic needle puncture at 23 days of gestation, and closed with either a platelet-enriched collagen plug with (n = 44) or without (n = 32) amniotic fluid cells. At 30 days of gestation, the defects were harvested and assessed microscopically. RESULTS: The plugs enriched with heterologous amniotic fluid cells more commonly had proliferating cells in the centre of the plug than those without cell addition. CFDA-SE labelling confirmed the presence of heterologous amniotic fluid cells over the entire membrane plug. Cell typing showed a mixture of fibroblasts and epithelial cells at the wound edges, whereas in the centre, there was an abundance of fibroblasts. CONCLUSION: When sealing iatrogenic membrane defects in the foetal rabbit model, enrichment of collagen plugs with platelets and amniotic fluid-derived heterologous foetal cells increases local cell proliferation.
Daisy Liekens; Liesbeth Lewi; Jacques Jani; Liesbeth Heyns; Eline Poliard; Godelieve Verbist; Nicole Ochsenbein-Kölble; Marc Hoylaerts; Jan Deprest
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  28     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-03     Completed Date:  2008-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-7     Citation Subset:  IM    
Interdepartmental Centre for Surgical Technologies, Faculty of Medicine and Department of Woman and Child, Division Woman, Laboratory Experimental Gynaecology, K.U. Leuven, Belgium.
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MeSH Terms
Amniotic Fluid / cytology*
Blood Platelets / physiology*
Cell Proliferation*
Disease Models, Animal
Extraembryonic Membranes / injuries*
Wound Healing / physiology*
Reg. No./Substance:
Comment In:
Prenat Diagn. 2008 Sep;28(9):876-7; author reply 878-80   [PMID:  18729136 ]

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