| Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila. | |
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MedLine Citation:
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PMID: 23150399 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The production of outer membrane vesicles (OMVs) is a widespread phenomenon employed by bacteria to secrete cell envelope components into the environment. A contribution of Legionella pneumophila OMVs to the pathogenesis of Legionnaires' disease is likely due to the high number of virulence-related proteins in the vesicles. OMVs are isolated from the supernatant of liquid cultures of L. pneumophila. After low-speed centrifugation, residual bacteria and cell fragments are eliminated by passing the supernatant through a filter. OMVs are pelleted by ultracentrifugation and resuspended in buffer. The isolated OMVs can be analyzed for their molecular components and their interactions with host structures, bacterial cells, or surfaces. |
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Authors:
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Jens Jäger; Michael Steinert |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 954 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 225-230 Citation Subset: - |
Affiliation:
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Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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