Document Detail

Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila.
MedLine Citation:
PMID:  23150399     Owner:  NLM     Status:  Publisher    
The production of outer membrane vesicles (OMVs) is a widespread phenomenon employed by bacteria to secrete cell envelope components into the environment. A contribution of Legionella pneumophila OMVs to the pathogenesis of Legionnaires' disease is likely due to the high number of virulence-related proteins in the vesicles. OMVs are isolated from the supernatant of liquid cultures of L. pneumophila. After low-speed centrifugation, residual bacteria and cell fragments are eliminated by passing the supernatant through a filter. OMVs are pelleted by ultracentrifugation and resuspended in buffer. The isolated OMVs can be analyzed for their molecular components and their interactions with host structures, bacterial cells, or surfaces.
Jens Jäger; Michael Steinert
Related Documents :
23658899 - Effect of dopamine on ehrlich ascites carcinoma cells.
20194639 - Integration of contractile forces during tissue invagination.
23800659 - Red cell indices: differentiation between β-thalassemia trait and iron deficiency anem...
23085529 - Trail protein localization in human primary t cells by 3d microscopy using 3d interacti...
23325239 - The rod pathway of the microbat retina has bistratified rod bipolar cells and tristrati...
8012449 - Immunocytochemical localization of annexins v and vi in human placentae of different ge...
6650659 - Tubulointerstitial inflammation, cast formation, and renal parenchymal damage in experi...
2265919 - Response persistence of cat retinal ganglion cells to the temporally discrete presentat...
2442859 - The application of propidium iodide staining to the study of the macronucleus and micro...
Publication Detail:
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  225-230     Citation Subset:  -    
Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Production of L. pneumophila Monospecies Biofilms in a Low-Nutrient-Concentration Medium.
Next Document:  Analysis of Legionella Infection by Flow Cytometry.