| Enrichment of multilocus sequence typing clade 1 with oral Candida albicans isolates in patients with untreated periodontitis. | |
MedLine Citation:
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PMID: 22875886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study investigated the prevalence and cell density of Candida species in periodontal pockets, healthy subgingival sites, and oral rinse samples of patients with untreated periodontitis. Twenty-one periodontitis patients underwent sampling at two periodontitis sites, and 19/21 of these patients underwent sampling at one periodontally healthy site. Both paper point and curette sampling techniques were employed. The periodontitis patients and 50 healthy subjects were also sampled by oral rinse. Candida isolates were recovered on CHROMagar Candida medium, and representative isolates were identified. Candida spp. were recovered from 10/21 (46.7%) periodontitis patients and from 16/50 (32%) healthy subjects. C. albicans predominated in both groups and was recovered from all Candida-positive subjects. Candida-positive periodontitis patients yielded Candida from periodontal pockets with average densities of 3,528 and 3,910 CFU/sample from curette and paper point samples, respectively, and 1,536 CFU/ml from oral rinse samples. The majority (18/19) of the healthy sites sampled from periodontitis patients were Candida negative. The 16 Candida-positive healthy subjects yielded an average of 279 CFU/ml from oral rinse samples. C. albicans isolates were investigated by multilocus sequence typing (MLST) to determine if specific clonal groups were associated with periodontitis. MLST analysis of 31 C. albicans isolates from periodontitis patients yielded 19 sequence types (STs), 13 of which were novel. Eleven STs belonged to MLST clade 1. In contrast, 16 C. albicans isolates from separate healthy subjects belonged to 16 STs, with 4 isolates belonging to clade 1. The distributions of STs between both groups were significantly different (P = 0.04) and indicated an enrichment of C. albicans isolates in periodontal pockets, which warrants a larger study. |
Authors:
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Brenda A McManus; Rory Maguire; Phillipa J Cashin; Noel Claffey; Stephen Flint; Mohammed H Abdulrahim; David C Coleman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-08-08 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 50 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-14 Completed Date: 2013-01-09 Revised Date: 2013-07-12 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 3335-44 Citation Subset: IM |
Affiliation:
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University of Dublin, Dublin, Republic of Ireland. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Candida albicans / classification*, genetics, isolation & purification* Candidiasis, Oral / microbiology* Cluster Analysis Colony Count, Microbial Female Genotype Humans Male Middle Aged Multilocus Sequence Typing* Mycological Typing Techniques Periodontal Pocket / microbiology Periodontitis / complications, microbiology* Specimen Handling / methods |
| Comments/Corrections | |
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