Document Detail


Enlarged and astaxanthin-accumulating cyst cells of the green alga Haematococcus pluvialis.
MedLine Citation:
PMID:  16233147     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The cyst cells of Haematococcus pluvialis were separated into fractions of relatively uniform size by sucrose density gradient centrifugation. The fraction at the bottom of the centrifuge tube with the largest specific gravity from density gradients of mature cysts mainly consisted of enlarged, red cyst cells and had the highest astaxanthin content. To examine the relationship between cell size and astaxanthin content of cysts, formation of the fluorescent dichlorofluorescein (DCF) from 2',7'-dichlorohydrofluorescein diacetate of cyst cells in each fraction from density-gradient centrifugation under oxidative stress caused by methyl viologen (1.0 mM) was studied. The formation of DCF in cyst cells was decreased with larger cell diameter. This decrease was also correlated with increases in astaxanthin content. Therefore, both cell diameter and the fluorescent DCF content of cyst cells would be good parameter to select astaxanthin-hyperproducing strains from native populations of H. pluvialis.
Authors:
M Kobayashi; T Katsuragi; Y Tani
Related Documents :
16643897 - Exogenous leukemia inhibitory factor (lif) attenuates retinal vascularization reducing ...
17628577 - Biochip for separating fetal cells from maternal circulation.
3216047 - Three-dimensional counting: an accurate and direct method to estimate numbers of cells ...
16972387 - Treatment of ballast water; how to test a system with a modular concept?
2409977 - In vitro translation of polyadenylated rna isolated from control and interferon-treated...
25242057 - Malpighian tubule development in the red flour beetle (tribolium castaneum).
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  92     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2001  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  565-8     Citation Subset:  -    
Affiliation:
Research Laboratory of Higashimaru Shoyu Co., Ltd., 100-3 Tominaga, Tatsuno, Hyogo 679-4193, Japan. mkobayashi@higashimaru.co.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Production of ethanol from liquefied cassava starch using co-immobilized cells of Zymomonas mobilis ...
Next Document:  Human N-acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and app...