| Enlarged and astaxanthin-accumulating cyst cells of the green alga Haematococcus pluvialis. | |
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MedLine Citation:
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PMID: 16233147 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The cyst cells of Haematococcus pluvialis were separated into fractions of relatively uniform size by sucrose density gradient centrifugation. The fraction at the bottom of the centrifuge tube with the largest specific gravity from density gradients of mature cysts mainly consisted of enlarged, red cyst cells and had the highest astaxanthin content. To examine the relationship between cell size and astaxanthin content of cysts, formation of the fluorescent dichlorofluorescein (DCF) from 2',7'-dichlorohydrofluorescein diacetate of cyst cells in each fraction from density-gradient centrifugation under oxidative stress caused by methyl viologen (1.0 mM) was studied. The formation of DCF in cyst cells was decreased with larger cell diameter. This decrease was also correlated with increases in astaxanthin content. Therefore, both cell diameter and the fluorescent DCF content of cyst cells would be good parameter to select astaxanthin-hyperproducing strains from native populations of H. pluvialis. |
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Authors:
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M Kobayashi; T Katsuragi; Y Tani |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 92 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2001 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 565-8 Citation Subset: - |
Affiliation:
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Research Laboratory of Higashimaru Shoyu Co., Ltd., 100-3 Tominaga, Tatsuno, Hyogo 679-4193, Japan. mkobayashi@higashimaru.co.jp |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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