Document Detail

Enhancing ultrasound-mediated cell membrane permeabilisation (sonoporation) using a high frequency pulse regime and implications for ultrasound-aided cancer chemotherapy.
MedLine Citation:
PMID:  18367324     Owner:  NLM     Status:  MEDLINE    
Delivering ultrasound to HeLa cells at 1MHz using a high frequency pulse regime (40kHz) and at a maximum energy density of 270Jcm(-2) resulted in significant cell membrane permeabilisation. Using FITC-dextran as a fluorogenic marker, optimally up to 64% of treated populations were permeabilised with cell viability remaining above 80%. Although cell membrane permeabilisation was observed in the presence of the microbubble-based ultrasound contrast agent, SonoVue, cell viability was severely compromised. Using the high frequency pulse regime in the absence of microbubbles, the LD50 of the cancer chemotherapeutic agent, camptothecin, was reduced from 58 to 18nM.
Ying Suet Li; Cherith N Reid; Anthony P McHale
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Publication Detail:
Type:  Journal Article     Date:  2008-03-25
Journal Detail:
Title:  Cancer letters     Volume:  266     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  156-62     Citation Subset:  IM    
School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine, Co. Derry BT52 1SA, Northern Ireland, United Kingdom.
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MeSH Terms
Antineoplastic Agents, Phytogenic / administration & dosage*,  metabolism,  toxicity
Camptothecin / administration & dosage*,  metabolism,  toxicity
Cell Membrane Permeability
Combined Modality Therapy
Dextrans / metabolism
Fluorescein-5-isothiocyanate / analogs & derivatives,  metabolism
Fluorescent Dyes / metabolism
Hela Cells
Neoplasms / drug therapy*,  therapy
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Fluorescent Dyes; 0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 7689-03-4/Camptothecin; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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