Document Detail


Enhancing polyphenol extraction from unripe apples by carbohydrate-hydrolyzing enzymes.
MedLine Citation:
PMID:  19946955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of process variables such as enzyme types, enzyme ratio, reaction temperature, pH, time, and ethanol concentration on the extraction of unripe apple polyphenol were investigated. The results indicated that Viscozyme L had the strongest effect on polyphenols extraction and was selected to study the polyphenol composition. The ratio of enzyme (Viscozyme L) to substrate (2 fungal beta-glucanase units (FBG)) at 0.02, reaction at pH 3.7, 50 degrees C for 12 h, and ethanol concentration of 70% were chosen as the most favorable extraction condition. Total phenolic content (TPC), reducing sugar content (RSC), and extraction yield increased by about 3, 1.5, and 2 times, respectively, compared with control. The contents of p-coumaric acid, ferulic acid, and caffeic acid increased to 8, 4, and 32 times, respectively. The enzyme-aided polyphenol extraction process from unripe apples might be applied to food industry for enhancing bioactive compound production.
Authors:
Hu-zhe Zheng; In-Wook Hwang; Shin-Kyo Chung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  10     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-30     Completed Date:  2010-03-01     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  912-9     Citation Subset:  IM    
Affiliation:
Department of Food Science and Technology, Kyungpook National University, Daegu 702-701, Korea.
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MeSH Terms
Descriptor/Qualifier:
Caffeic Acids / chemistry
Carbohydrates / chemistry*
Chlorogenic Acid / chemistry
Coumaric Acids / chemistry
Ethanol / chemistry
Flavonoids / chemistry*
Glucans / chemistry*
Glycoside Hydrolases / chemistry*
Hydrogen-Ion Concentration
Hydrolysis
Malus / enzymology*
Phenol / chemistry
Phenols / chemistry*
Protein Binding
Temperature
Chemical
Reg. No./Substance:
0/Caffeic Acids; 0/Carbohydrates; 0/Coumaric Acids; 0/Flavonoids; 0/Glucans; 0/Phenols; 0/polyphenols; 108-95-2/Phenol; 1135-24-6/ferulic acid; 327-97-9/Chlorogenic Acid; 331-39-5/caffeic acid; 64-17-5/Ethanol; 7400-08-0/4-coumaric acid; EC 3.2.1.-/Glycoside Hydrolases
Comments/Corrections

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